[HTML][HTML] PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Background The rapid expansion of the CRISPR toolbox through tagging effector domains
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology
LJ Zhu - Frontiers in Biology, 2015 - Springer
Abstract CRISPR-Cas (Clustered, Regularly Interspaced, Short Palindromic Repeats–
CRISPR-associated (Cas)) RNA guided endonuclease has emerged as the most effective …
CRISPR-associated (Cas)) RNA guided endonuclease has emerged as the most effective …
CRISPR-RT: a web application for designing CRISPR-C2c2 crRNA with improved target specificity
CRISPR-Cas systems have been successfully applied in genome editing. Recently, the
CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe …
CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe …
beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing
The biological relevance of the present method pertains to CRISPR-mediated base editing
technology that has opened new avenues for scar-free genome-wide mutagenesis and thus …
technology that has opened new avenues for scar-free genome-wide mutagenesis and thus …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
A quick guide to CRISPR sgRNA design tools
VA Brazelton Jr, S Zarecor, DA Wright, Y Wang… - GM crops & …, 2015 - Taylor & Francis
Targeted genome editing is now possible in nearly any organism and is widely
acknowledged as a biotech game-changer. Among available gene editing techniques, the …
acknowledged as a biotech game-changer. Among available gene editing techniques, the …
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
[HTML][HTML] Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
[HTML][HTML] Minimizing off-target effects in CRISPR-Cas9 genome editing
SJ Chen - Cell biology and toxicology, 2019 - Springer
CRISPR (clustered regularly interspaced short palindromic repeats), originally an antiviral
immune system adopted by bacteria and archaea, is repurposed and developed into a …
immune system adopted by bacteria and archaea, is repurposed and developed into a …
A highly specific SpCas9 variant is identified by in vivo screening in yeast
A Casini, M Olivieri, G Petris, C Montagna… - Nature …, 2018 - nature.com
Despite the utility of CRISPR–Cas9 nucleases for genome editing, the potential for off-target
activity limits their application, especially for therapeutic purposes,. We developed a yeast …
activity limits their application, especially for therapeutic purposes,. We developed a yeast …