Biosynthesis of a novel fisetin glycoside from engineered Escherichia coli

RP Pandey, P Parajuli, LL Chu, SY Kim… - Journal of Industrial and …, 2016 - Elsevier
Abstract Escherichia coli BL21 (DE3)/ΔpgiΔzwfΔgalU mutant was engineered by
overexpressing thymidine diphosphate (dTDP)-d-glucose synthase (tgs), dTDP-d-glucose 4 …

Fisetin glycosides synthesized by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01: characterization, molecular docking, and antioxidant activity

N Lorthongpanich, P Mahalapbutr, T Rungrotmongkol… - PeerJ, 2022 - peerj.com
Fisetin is a flavonoid that exhibits high antioxidant activity and is widely employed in the
pharmacological industries. However, the application of fisetin is limited due to its low water …

Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli

P Parajuli, RP Pandey, NTH Trang, AK Chaudhary… - Microbial cell …, 2015 - Springer
Background A multi-monocistronic synthetic vector was used to assemble multiple genes of
a nucleotide diphosphate (NDP)-sugar biosynthetic pathway to construct robust genetic …

[HTML][HTML] Biochemical characterization of synthesized fisetin glucoside by dextransucrase from Leuconostoc mesenteroides NRRL B-1299CB4 with enhanced water …

Y Moon, H Kim, CG Kang, C Park, SW Kim… - Enzyme and Microbial …, 2022 - Elsevier
Abstract Fisetin (7, 3', 4'-flavon-3-ol) is a flavonol found in plants, fruits, and vegetables. It
exhibits diverse biological activities, including antioxidant, anti-inflammatory, and anti …

Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli

RP Pandey, P Parajuli, LL Chu, S Darsandhari… - Biochemical …, 2015 - Elsevier
Abstract Escherichia coli BL21 (DE3) was engineered to divert the flow of carbon flux from
glucose-1-phosphate to thymidine diphosphate 4-keto 4, 6-dideoxy-D-glucose (dTKDG), an …

Biosynthesis of flavone C-glucosides in engineered Escherichia coli

A Shrestha, RP Pandey, D Dhakal, P Parajuli… - Applied microbiology …, 2018 - Springer
Two plant-originated C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and
GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones …

Highly efficient biosynthesis of glycyrrhetinic acid glucosides by coupling of microbial glycosyltransferase to plant sucrose synthase

MY Ali, Q Chang, Q Yan, Z Qian, X Guo… - … in Bioengineering and …, 2021 - frontiersin.org
Glycyrrhetinic acid (GA) is a principal bioactive pentacyclic triterpenoid from Glycyrrhiza
uralensis. Uridine diphosphate-dependent glycosyltransferases (UGTs) have been widely …

Identification of a permissive glycosyltransferase UGT73AC11 for flavonoids glycosylation

X Liu, W Xu, J Li, X Feng, C Li - Biochemical Engineering Journal, 2024 - Elsevier
Flavonoids, natural products derived from plants, have shown promising potential in food
and medicine. These compounds are commonly found in the form of glycosides, which …

[HTML][HTML] Glucosylation of isoflavonoids in engineered Escherichia coli

RP Pandey, P Parajuli, N Koirala, JH Lee, YI Park… - Molecules and cells, 2014 - Elsevier
A glycosyltransferase, YjiC, from Bacillus licheniformis has been used for the modification of
the commercially available isoflavonoids genistein, daidzein, biochanin A and formononetin …

Engineering Escherichia coli MG1655 for Highly Efficient Biosynthesis of 2′-Fucosyllactose by De Novo GDP-Fucose Pathway

R Chen, Y Zhu, H Wang, Y Liu, J Meng… - Journal of Agricultural …, 2023 - ACS Publications
2′-Fucosyllactose (2′-FL), the most typical human milk oligosaccharide, is used as an
additive in premium infant formula. Herein, we constructed two highly effective 2′-FL …