Cryo-electron tomography and subtomogram averaging are rising and fast-evolving imaging
techniques to study biological events, providing structural information at an unprecedented …

Highlights•Cryo-ET and subtomogram averaging allow visualizing macromolecular
complexes in close to physiological conditions.•This offers a significant potential for in situ …

Cryo-electron tomography combined with image processing by sub-tomogram averaging is
unique in its power to resolve the structures of proteins and macromolecular complexes in …

Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex
biological specimens such as viral particles, cellular sections and, in some cases, whole …

Cryo-electron tomography provides low-resolution 3D views of cells, organelles, or viruses.
Macromolecular complexes present in multiple copies can be subsequently identified within …

Electron microscopy (EM) techniques have been crucial for understanding the structure of
biological specimens such as cells, tissues and macromolecular assemblies. Viruses and …

The combination of cryo-electron tomography and subtomogram analysis affords 3D high-
resolution views of biological macromolecules in their native cellular environment, or in situ …

Highlights•Cryo-electron tomography (cryoET) subtomogram averaging has emerged as a
structural biology method for sparse and heterogenerous sampls.•CryoET subtomogram …

Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in
recent years. It provides structures of macromolecular complexes in situ and in cellular …

A wide range of medically important nanosized biological assemblies are not amenable to
study by standard structural techniques, such as x-ray crystallography or nuclear magnetic …