Analysis by polymerase chain reaction of the physical state of human papillomavirus type 16 DNA in cervical preneoplastic and neoplastic lesions

BC Das, JK Sharma… - Journal of General …, 1992 - microbiologyresearch.org
Integration of human papillomavirus (HPV) DNA into the host cell genome is believed to be
essential for malignant progression. However unambiguous detection of the physical state of …

Analysis of the physical state of different human papillomavirus DNAs in intraepithelial and invasive cervical neoplasm

AP Cullen, R Reid, M Campion, AT Lörincz - Journal of virology, 1991 - Am Soc Microbiol
The integration of human papillomavirus (HPV) DNA into the human genome has been
generally accepted as a characteristic of malignant lesions. To gain a better understanding …

Thes physical state of human papillomavirus 16 DNAxs in cervical carcinoma and cervical intraepithelial neoplasia

M Fukushima, Y Yamakawa, S Shimano… - Cancer, 1990 - Wiley Online Library
Cervical carcinomas and cervical intraepithelial neoplasias (CIN) were analyzed for the
presence of human papillomavirus (HPV) DNA using Southern blot hybridization. of the five …

Analysis by multiplex PCR of the physical status of human papillomavirus type 16 DNA in cervical cancers

M Yoshinouchi, A Hongo, K Nakamura… - Journal of clinical …, 1999 - Am Soc Microbiol
Integration of human papillomavirus (HPV) DNA occurs early in cancer development and is
an important event in malignant transformation of cervical cancer. Integration of HPVs …

Detection of human papillomavirus type 16 DNA and evidence for integration into the cell DNA in cervical dysplasia

H Shirasawa, Y Tomita, K Kubota… - Journal of general …, 1986 - microbiologyresearch.org
The presence of human papillomavirus (HPV) type 16 DNA in biopsies from precancerous
lesions and from early lesions of human cervical cancer, and the integration of virus DNA …

Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR

S Nagao, M Yoshinouchi, Y Miyagi… - Journal of clinical …, 2002 - Am Soc Microbiol
ABSTRACT A rapid quantitative real-time PCR method was employed to quantify the copy
number of E2 and E6 genes for analysis of the physical status of human papillomavirus type …

Physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract

H Lehn, LL Villa, F Marziona… - Journal of General …, 1988 - microbiologyresearch.org
The DNA of distinct human papillomaviruses (HPVs) is regularly detected in the majority of
human cervical carcinomas. In contrast to benign HPV-induced genital lesions, where the …

Analysis of integrated human papillomavirus type 16 DNA in cervical cancers: amplification of viral sequences together with cellular flanking sequences

M Wagatsuma, K Hashimoto, T Matsukura - Journal of virology, 1990 - Am Soc Microbiol
We have isolated four clones of integrated human papillomavirus type 16 (HPV-16) DNA
from four different primary cervical cancer specimens. All clones were found to be …

Comparison between in situ hybridization and real-time PCR technique as a means of detecting the integrated form of human papillomavirus 16 in cervical neoplasia

T Fujii, N Masumoto, M Saito, N Hirao… - Diagnostic Molecular …, 2005 - journals.lww.com
Integration of the human papillomavirus (HPV) genome is thought to be one of the causes of
cancer progression. However, there is controversy concerning the physical status of HPV 16 …

Physical state of HPV16 and chromosomal mapping of the integrated form in cervical carcinomas

M Kalantari, E Blennow, B Hagmar… - Diagnostic Molecular …, 2001 - journals.lww.com
Using a procedure based on restriction enzyme cleavage, self-ligation, and inverse
polymerase chain reaction (rli PCR), the authors investigated 18 cervical intraepithelial …