This communication describes the establishment of the type II bacterial CRISPR–Cas9
system to efficiently disrupt target genes in the fungal maize pathogen Ustilago maydis. A …

The rice blast fungus Magnaporthe oryzae is the most serious pathogen of cultivated rice
and a significant threat to global food security. To accelerate targeted mutation and specific …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …

Functional genomics of filamentous fungi has gradually uncovered gene information for
constructing 'cell factories' and controlling pathogens. Available gene manipulation methods …

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

Objectives To develop a genome editing method using the CRISPR/Cas9 system in
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …

CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome
editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools …

CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated
proteins) is an adaptive immune system in bacteria and archaea to defend against invasion …