Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes
Synthetic alkane-inducible biosensors have applications as detectors for environmental
hydrocarbon contamination and as novel inducible expression systems with low-cost …
hydrocarbon contamination and as novel inducible expression systems with low-cost …
Twin-layer biosensor for real-time monitoring of alkane metabolism
Intracellular metabolic sensors can be used for efficient screening and optimization of
microbial cell factories. In particular, the sensors are useful in acquiring information about …
microbial cell factories. In particular, the sensors are useful in acquiring information about …
Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
W Wu, L Zhang, L Yao, X Tan, X Liu, X Lu - Scientific reports, 2015 - nature.com
Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is
severely limited due to the lack of a fast detection method against alkanes. Here we first …
severely limited due to the lack of a fast detection method against alkanes. Here we first …
Directly evolved AlkS-based biosensor platform for monitoring and high-throughput screening of alkane production
D Chen, S Xu, S Li, S Tao, L Li, S Chen… - ACS Synthetic …, 2023 - ACS Publications
Biosynthetic alkane using acyl-ACP aldehyde reductase (AAR) and aldehyde-deformylating
oxygenase (ADO) from cyanobacteria is considered a promising alternative for the …
oxygenase (ADO) from cyanobacteria is considered a promising alternative for the …
Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes
C Grant, D Deszcz, YC Wei, RJ MartInez-Torres… - Scientific reports, 2014 - nature.com
Effective application of whole-cell devices in synthetic biology and biocatalysis will always
require consideration of the uptake of molecules of interest into the cell. Here we …
require consideration of the uptake of molecules of interest into the cell. Here we …
Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators
JF Juárez, B Lecube-Azpeitia, SL Brown… - Nature …, 2018 - nature.com
The ability of bacteria to sense specific molecules within their environment and trigger
metabolic responses in accordance is an invaluable biotechnological resource. While many …
metabolic responses in accordance is an invaluable biotechnological resource. While many …
Strategies for improving small-molecule biosensors in bacteria
In recent years, small-molecule biosensors have become increasingly important in synthetic
biology and biochemistry, with numerous new applications continuing to be developed …
biology and biochemistry, with numerous new applications continuing to be developed …
[HTML][HTML] A unified design allows fine-tuning of biosensor parameters and application across bacterial species
CK Sonntag, LK Flachbart, C Maass, M Vogt… - Metabolic engineering …, 2020 - Elsevier
In recent years, transcriptional biosensors have become valuable tools in metabolic
engineering as they allow semiquantitative determination of metabolites in single cells …
engineering as they allow semiquantitative determination of metabolites in single cells …
An intracellular sensing and signal transduction system that regulates the metabolism of polycyclic aromatic hydrocarbons in bacteria
W Wang, Z Shao - Msystems, 2021 - Am Soc Microbiol
Many bacteria utilize polycyclic aromatic hydrocarbon (PAH) as carbon and energy sources
for growth. These bacteria play an important role in the amelioration of PAH pollution in …
for growth. These bacteria play an important role in the amelioration of PAH pollution in …
A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution
RK Jha, JM Bingen, CW Johnson, TL Kern… - Metabolic engineering …, 2018 - Elsevier
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput
strain improvement in synthetic biology. Many biosensors are developed in model …
strain improvement in synthetic biology. Many biosensors are developed in model …