Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology
applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we …

Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes,
particularly when screened in multiple growth conditions. Here, we screened a pooled …

Trans-acting regulators provide novel opportunities to study essential genes and regulate
metabolic pathways. We have adapted the clustered regularly interspersed palindromic …

Cyanobacteria are photoautotrophic prokaryotes that serve as key model organisms to study
basic photosynthetic processes and are potential carbon-negative production chassis for …

Abstract Background Cyanobacterium Synechococcus elongatus PCC 7942 holds promise
for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be …

Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower
volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures …

In metabolic engineering, the production of industrially relevant chemicals, via rational
engineering of microorganisms, is an intensive area of research. One particular group of …

We describe the application of clustered regularly interspaced short palindromic repeats
interference (CRISPRi) for gene repression in the model cyanobacterium Synechcocystis sp …

Recent advances in synthetic biology and an emerging algal biotechnology market have
spurred a prolific increase in the availability of molecular tools for cyanobacterial research …

In cyanobacteria, metabolic engineering using synthetic biology tools is limited to build a
biosolar cell factory that converts CO2 to value-added chemicals, as repression of essential …