Digital PCR: a reliable tool for analyzing and monitoring hematologic malignancies

N Coccaro, G Tota, L Anelli, A Zagaria… - International journal of …, 2020 - mdpi.com
The digital polymerase chain reaction (dPCR) is considered to be the third-generation
polymerase chain reaction (PCR), as it yields direct, absolute and precise measures of …

Digital PCR in myeloid malignancies: ready to replace quantitative PCR?

D Cilloni, J Petiti, V Rosso, G Andreani… - International journal of …, 2019 - mdpi.com
New techniques are on the horizon for the detection of small leukemic clones in both, acute
leukemias and myeloproliferative disorders. A promising approach is based on digital …

[HTML][HTML] Medical diagnostic value of digital PCR (dPCR): A systematic review

S Nazir - Biomedical Engineering Advances, 2023 - Elsevier
Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute
quantification of target nucleic acids. dPCR got attention as a precise quantification tool in …

Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications

L Cao, X Cui, J Hu, Z Li, JR Choi, Q Yang, M Lin… - Biosensors and …, 2017 - Elsevier
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a
significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and …

[HTML][HTML] Principles of digital PCR and its applications in current obstetrical and gynecological diseases

X Mao, C Liu, H Tong, Y Chen, K Liu - American journal of …, 2019 - ncbi.nlm.nih.gov
Digital PCR (dPCR) is a revolutionary technique to precisely quantify nucleic acids. For its
high sensitivity and specificity, this technique has been widely replicated worldwide. To …

Principle and applications of digital PCR

G Pohl, IM Shih - Expert review of molecular diagnostics, 2004 - Taylor & Francis
Digital PCR represents an example of the power of PCR and provides unprecedented
opportunities for molecular genetic analysis in cancer. The technique is to amplify a single …

Digital droplet PCR in hematologic malignancies: a new useful molecular tool

S Galimberti, S Balducci, F Guerrini, M Del Re… - Diagnostics, 2022 - mdpi.com
Digital droplet PCR (ddPCR) is a recent version of quantitative PCR (QT-PCR), useful for
measuring gene expression, doing clonality assays and detecting hot spot mutations. In …

Considerations for digital PCR as an accurate molecular diagnostic tool

JF Huggett, S Cowen, CA Foy - Clinical chemistry, 2015 - academic.oup.com
Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …

Basic concepts and validation of digital PCR measurements

L Pinheiro, KR Emslie - Digital PCR: Methods and Protocols, 2018 - Springer
Use of digital polymerase chain reaction (dPCR) technology is rapidly growing and
diversifying into a range of areas in life science. The release of dPCR commercial systems …

Digital assays part I: partitioning statistics and digital PCR

AS Basu - SLAS TECHNOLOGY: Translating Life Sciences …, 2017 - journals.sagepub.com
A digital assay is one in which the sample is partitioned into many small containers such that
each partition contains a discrete number of biological entities (0, 1, 2, 3,…). A powerful …