Multiscale cytometry and regulation of 3D cell cultures on a chip
Three-dimensional cell culture is emerging as a more relevant alternative to the traditional
two-dimensional format. Yet the ability to perform cytometry at the single cell level on intact …
two-dimensional format. Yet the ability to perform cytometry at the single cell level on intact …
Individual control and quantification of 3D spheroids in a high-density microfluidic droplet array
As three-dimensional cell culture formats gain in popularity, there emerges a need for tools
that produce vast amounts of data on individual cells within the spheroids or organoids …
that produce vast amounts of data on individual cells within the spheroids or organoids …
Microfluidic 3D cell culture: potential application for tissue-based bioassays
Current fundamental investigations of human biology and the development of therapeutic
drugs commonly rely on 2D monolayer cell culture systems. However, 2D cell culture …
drugs commonly rely on 2D monolayer cell culture systems. However, 2D cell culture …
Three-dimensional cell culture and drug testing in a microfluidic sidewall-attached droplet array
SP Zhao, Y Ma, Q Lou, H Zhu, B Yang… - Analytical …, 2017 - ACS Publications
Three-dimensional (3D) cell culture provides an effective way over conventional two-
dimensional (2D) monolayer culture to more closely imitate the complex cellular …
dimensional (2D) monolayer culture to more closely imitate the complex cellular …
Spheroid engineering in microfluidic devices
Two-dimensional (2D) cell culture techniques are commonly employed to investigate
biophysical and biochemical cellular responses. However, these culture methods, having …
biophysical and biochemical cellular responses. However, these culture methods, having …
A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
AR Thomsen, C Aldrian, P Bronsert, Y Thomann… - Lab on a chip, 2018 - pubs.rsc.org
Multicellular spheroids represent a well-established 3D model to study healthy and
diseased cells in vitro. The use of conventional 3D cell culture platforms for the generation of …
diseased cells in vitro. The use of conventional 3D cell culture platforms for the generation of …
From 3D cell culture to organs-on-chips
3D cell-culture models have recently garnered great attention because they often promote
levels of cell differentiation and tissue organization not possible in conventional 2D culture …
levels of cell differentiation and tissue organization not possible in conventional 2D culture …
High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform
Guided assembly of microscale tissue subunits (ie 3D cell clusters/aggregates) has found
applications in cell therapy/tissue engineering, cell and developmental biology, and drug …
applications in cell therapy/tissue engineering, cell and developmental biology, and drug …
Current strategies with implementation of three-dimensional cell culture: the challenge of quantification
J Temple, E Velliou, M Shehata, R Lévy… - Interface …, 2022 - royalsocietypublishing.org
From growing cells in spheroids to arranging them on complex engineered scaffolds, three-
dimensional cell culture protocols are rapidly expanding and diversifying. While these …
dimensional cell culture protocols are rapidly expanding and diversifying. While these …
Is it time to start transitioning from 2D to 3D cell culture?
Cell culture is an important and necessary process in drug discovery, cancer research, as
well as stem cell study. Most cells are currently cultured using two-dimensional (2D) …
well as stem cell study. Most cells are currently cultured using two-dimensional (2D) …