Abstract CRISPR/Cas9-mediated homology-directed recombination is an efficient method to
express target genes. Based on the above method, providing ideal neutral integration sites …

Background Targeted DNA integration is widely used in basic research and commercial
applications because it eliminates positional effects on transgene expression. Targeted …

Fusarium oxysporum is an economically important pathogen that widely exists in the
environment and is capable of causing serious problems in crop production and …

Genome editing using the clustered regularly interspaced short palindromic repeats
(CRISPR)-CRISPR-associated protein 9 (Cas9) system has greatly facilitated the genetic …

Background Besides their ability to produce several interesting bioactive secondary
metabolites, members of the Fusarium solani species complex comprise important …

AbstractBackground and ObjectivesA clustered regularly interspaced short palindromic
repeat (CRISPR) is one of the precise genome editing techniques. In present experiment …

CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed
Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and …

ABSTRACT CRISPR/Cas‐derived RNA‐guided nucleases (RGNs) that can generate DNA
double‐strand breaks (DSBs) at a specific sequence are widely used for targeted genome …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9)-mediated genome editing has become a promising approach for efficient …

Integrating a desired DNA sequence into the yeast genomes is a widely-used genetic
manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration …