Design of heme enzymes with a tunable substrate binding pocket adjacent to an open metal coordination site
The catalytic versatility of pentacoordinated iron is highlighted by the broad range of natural
and engineered activities of heme enzymes such as cytochrome P450s, which position a …
and engineered activities of heme enzymes such as cytochrome P450s, which position a …
An evolved orthogonal enzyme/cofactor pair
EW Reynolds, MW McHenry, F Cannac… - Journal of the …, 2016 - ACS Publications
We introduce a strategy that expands the functionality of hemoproteins through orthogonal
enzyme/heme pairs. By exploiting the ability of a natural heme transport protein, ChuA, to …
enzyme/heme pairs. By exploiting the ability of a natural heme transport protein, ChuA, to …
Selective CH bond functionalization with engineered heme proteins: new tools to generate complexity
Highlights•Interplay between synthetic chemistry and enzyme discovery/creation.•
Cytochromes P450 for selective hydroxylation of complex molecules.•Engineered heme …
Cytochromes P450 for selective hydroxylation of complex molecules.•Engineered heme …
A chemically programmed proximal ligand enhances the catalytic properties of a heme enzyme
Enzymes rely on complex interactions between precisely positioned active site residues as a
mechanism to compensate for the limited functionality contained within the genetic code …
mechanism to compensate for the limited functionality contained within the genetic code …
A de novo peroxidase is also a promiscuous yet stereoselective carbene transferase
R Stenner, JW Steventon, A Seddon… - Proceedings of the …, 2020 - National Acad Sciences
By constructing an in vivo-assembled, catalytically proficient peroxidase, C45, we have
recently demonstrated the catalytic potential of simple, de novo-designed heme proteins …
recently demonstrated the catalytic potential of simple, de novo-designed heme proteins …
Navigating the unnatural reaction space: directed evolution of heme proteins for selective carbene and nitrene transfer
Conspectus Despite the astonishing diversity of naturally occurring biocatalytic processes,
enzymes do not catalyze many of the transformations favored by synthetic chemists. Either …
enzymes do not catalyze many of the transformations favored by synthetic chemists. Either …
Mimicking heme enzymes in the solid state: metal–organic materials with selectively encapsulated heme
To carry out essential life processes, nature has had to evolve heme enzymes capable of
synthesizing and manipulating complex molecules. These proteins perform a plethora of …
synthesizing and manipulating complex molecules. These proteins perform a plethora of …
Designer heme proteins: Achieving novel function with abiological heme analogues
CM Lemon, MA Marletta - Accounts of chemical research, 2021 - ACS Publications
Conspectus Heme proteins have proven to be a convenient platform for the development of
designer proteins with novel functionalities. This is achieved by substituting the native iron …
designer proteins with novel functionalities. This is achieved by substituting the native iron …
Stereoselective enzymatic synthesis of heteroatom-substituted cyclopropanes
The repurposing of hemoproteins for non-natural carbene transfer activities has generated
enzymes for functions previously accessible only to chemical catalysts. With activities …
enzymes for functions previously accessible only to chemical catalysts. With activities …
Artificial diiron enzymes with a de novo designed four‐helix bundle structure
A single polypeptide chain may provide an astronomical number of conformers. Nature
selected only a trivial number of them through evolution, composing an alphabet of …
selected only a trivial number of them through evolution, composing an alphabet of …