Improved harvest and fixation methodology for isolated human peripheral blood mononuclear cells in cytokinesis-block micronucleus assay

VST Goh, R Nakayama, WF Blakely, Y Abe… - … Journal of Radiation …, 2021 - Taylor & Francis
Purpose In suspected radiation exposures, cytokinesis-block micronucleus (CBMN) assay is
used for biodosimetry by detecting micronuclei (MN) in binucleated (BN) cells in whole …

Cytokinesis-block micronucleus assay performed in 0 and 2 Gy irradiated whole blood and isolated PBMCs in a six-well transwell co-culture system

VST Goh, K Takebayashi, R Nakayama… - … Journal of Radiation …, 2021 - Taylor & Francis
Purpose Cytokinesis-block micronucleus (CBMN) assay in cytogenetic biodosimetry uses
micronucleus (MN) frequency scored in binucleated cells (BNC) for dose estimation. Cell …

Manual scoring with shortened 48 h cytokinesis-block micronucleus assay feasible for triage in the event of a mass-casualty radiation accident

VS Ting Goh, Y Fujishima, R Nakayama… - Radiation …, 2023 - meridian.allenpress.com
The cytokinesis-block micronucleus (CBMN) assay in cytogenetic biodosimetry uses
micronucleus (MN) frequency scored in binucleated cells (BNCs) to estimate ionizing …

Adaption of the cytokinesis-block micronucleus cytome assay for improved triage biodosimetry

C Beinke, M Port, A Riecke, CG Ruf… - Radiation …, 2016 - meridian.allenpress.com
The purpose of this work was to adapt a more advanced form of the cytokinesis-block
micronucleus (CBMN) cytome assay for triage biodosimetry in the event of a mass casualty …

Optimized automated data analysis for the cytokinesis‐block micronucleus assay using imaging flow cytometry for high throughput radiation biodosimetry

MA Rodrigues, CE Probst, LA Beaton‐Green… - Cytometry Part …, 2016 - Wiley Online Library
The cytokinesis‐block micronucleus (CBMN) assay is a well‐established technique that can
be employed in triage radiation biodosimetry to estimate whole body doses of radiation to …

The cytokinesis-block micronucleus assay on human isolated fresh and cryopreserved peripheral blood mononuclear cells

S Sioen, K Cloet, A Vral, A Baeyens - Journal of Personalized Medicine, 2020 - mdpi.com
The cytokinesis-block micronucleus (CBMN) assay is a standardized method used for
genotoxicity studies. Conventional whole blood cultures (WBC) are often used for this assay …

Cytokinesis blocked micronucleus (CB-MN) assay for biodosimetry of high dose accidental exposure

K Shirsath, N Bhat, K Anjaria, U Desai… - 2014 - sid.ir
Background: Most of the cytogenetic dosimetry, except Premature Chromosome
Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of …

Development of a high-throughput and miniaturized cytokinesis-block micronucleus assay for use as a biological dosimetry population triage tool

SW Lue, M Repin, R Mahnke… - Radiation …, 2015 - meridian.allenpress.com
Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For
a large mass-casualty event involving exposure to ionizing radiation, it is of utmost …

[PDF][PDF] Cytokinesis-block micronucleus (CBMN) assay/CBMN cytome assay in human lymphocytes after in vitro irradiation and its use in biodosimetry

J Pejchal, V Vasilieva, M Hristozova, Z Vilasová… - Mil Med Sci …, 2011 - academia.edu
Aim: Cytokinesis-block micronucleus (CBMN) assay and its comprehensive variant CBMN
cytome assay are cytogenetic methods. CBMN is based on assessment of micronuclei in …

Multi‐parameter dose estimations in radiation biodosimetry using the automated cytokinesis‐block micronucleus assay with imaging flow cytometry

MA Rodrigues, LA Beaton‐Green, BC Kutzner… - Cytometry Part …, 2014 - Wiley Online Library
The cytokinesis‐block micronucleus (CBMN) assay is an established technique in radiation
biological dosimetry for estimating the dose to an individual by measuring the frequency of …