Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture

Y Li, C Liu, F Bai, X Zhao - Bioresource technology, 2016 - Elsevier
Cellulase is a prerequisite for the bioconversion of lignocellulosic biomass, but its high cost
presents the biggest challenge. In this article, low-cost mixture was produced from glucose …

Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

Y Li, P Zhang, D Zhu, B Yao, T Hasunuma… - Biochemical …, 2022 - Elsevier
Although high-level cellulase production by Trichoderma reesei from lignocellulosic
biomass in biorefinery has been widely studied, more efficient and economic hydrolysis is …

Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei

J Gao, Y Qian, Y Wang, Y Qu, Y Zhong - Biotechnology for biofuels, 2017 - Springer
Background The enzymes for efficient hydrolysis of lignocellulosic biomass are a major
factor in the development of an economically feasible cellulose bioconversion process. Up …

On-site cellulase production and efficient saccharification of corn stover employing cbh2 overexpressing Trichoderma reesei with novel induction system

Y Li, X Zhang, L Xiong, MA Mehmood, X Zhao… - Bioresource …, 2017 - Elsevier
Although on-site cellulase production offers cost-effective saccharification of lignocellulosic
biomass, low enzyme titer is still a barrier for achieving robustness. In the present study, a …

Engineering Trichoderma reesei for Hyperproduction of Cellulases on Glucose to Efficiently Saccharify Pretreated Corncobs

F Zheng, R Yang, Y Cao, W Zhang, X Lv… - Journal of Agricultural …, 2020 - ACS Publications
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is widely used
as a cellulase producer in the industry. Herein, we describe the rational engineering of the …

Cellulase hyper-production by Trichoderma reesei mutant SEU-7 on lactose

C Li, F Lin, L Zhou, L Qin, B Li, Z Zhou, M Jin… - Biotechnology for …, 2017 - Springer
Background The induction of cellulase production by insoluble carbon source cellulose was
a common and efficient strategy, but has some drawbacks, such as difficult fermentation …

Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose

S Raghuwanshi, D Deswal, M Karp, RC Kuhad - Fuel, 2014 - Elsevier
A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation
for enhanced cellulase production and lower catabolite repression. The production of …

Cellulase production by recombinant Trichoderma reesei and its application in enzymatic hydrolysis of agricultural residues

H Fang, L Xia - Fuel, 2015 - Elsevier
Fed-batch fermentation was employed to improve the productivity of Trichoderma reesei
cellulase. The optimal conditions were fed-batch initiating time 48 h, lactose concentration …

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Y Zhang, J Yang, L Luo, E Wang, R Wang… - International journal of …, 2020 - mdpi.com
Fermentable sugars are important intermediate products in the conversion of lignocellulosic
biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the …

Enhanced cellulase and reducing sugar production by a new mutant strain Trichoderma harzianum EUA20

H Wang, L Zhai, A Geng - Journal of bioscience and bioengineering, 2020 - Elsevier
Trichoderma harzianum EU2-77 was a mutant strain of the wild-type strain T. harzianum
NP13a isolated in Singapore. A multi-mutagenesis one-screening (MMOS) method was …