Splicing of mRNA precursors is essential in the regulation of gene expression. U2AF65
recognizes the poly-pyrimidine tract and helps in the recognition of the branch point …

Recognition of polypyrimidine (Py) tracts typically present between the branch point and the
3′ splice site by the large subunit of the essential splicing factor U2AF is a key early step in …

Exons are ligated in an ordered manner without the skipping of exons in the constitutive
splicing of pre-mRNAs with multiple introns. To identify factors ensuring ordered exon …

Removal of pre-mRNA introns is an essential step in eukaryotic genome interpretation. The
spliceosome, a ribonucleoprotein performs this critical function; however, precise roles for …

Pre-mRNA splicing is accomplished by the spliceosome, a megadalton complex that
assembles de novo on each intron. Because spliceosome assembly and catalysis occur …

U2 snRNA-intron branchpoint pairing is a critical step in pre-mRNA recognition by the
splicing apparatus, but the mechanism by which these two RNAs engage each other is …

The fission yeast genome, which contains numerous short introns, is an apt model for
studies on fungal splicing mechanisms and splicing by intron definition. Here we perform a …

Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and
introns are removed from pre-mRNA by the spliceosome. Understanding the time lag …

Intron diversity facilitates regulated gene expression and alternative splicing. Spliceosomes
excise introns after recognizing their splicing signals: the 5′-splice site (5′ ss) …

Pre‐mRNA splicing has to be coordinated with other processes occurring in the nucleus
including transcription, mRNA 3′ end formation and mRNA export. To analyze the …