Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology

I Vojnovic, J Winkelmeier… - Biochemical Society …, 2019 - portlandpress.com
In this review, we discuss multi-color single-molecule imaging and tracking strategies for
studying microbial cell biology. We first summarize and compare the methods in a detailed …

[PDF][PDF] Rediscovering bacteria through single-molecule imaging in living cells

AN Kapanidis, A Lepore, M El Karoui - Biophysical Journal, 2018 - cell.com
Bacteria are microorganisms central to health and disease, serving as important model
systems for our understanding of molecular mechanisms and for developing new …

From single bacterial cell imaging towards in vivo single-molecule biochemistry studies

U Endesfelder - Essays in Biochemistry, 2019 - portlandpress.com
Bacteria as single-cell organisms are important model systems to study cellular mechanisms
and functions. In recent years and with the help of advanced fluorescence microscopy …

New fluorescence microscopy methods for microbiology: sharper, faster, and quantitative

Z Gitai - Current opinion in microbiology, 2009 - Elsevier
In addition to the inherent interest stemming from their ecological and human health impacts,
microbes have many advantages as model organisms, including ease of growth and …

Progress and prospects for small-molecule probes of bacterial imaging

O Kocaoglu, EE Carlson - Nature chemical biology, 2016 - nature.com
Fluorescence microscopy is an essential tool for the exploration of cell growth, division,
transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid …

Shedding light on biology of bacterial cells

JP Schneider, M Basler - Philosophical Transactions of …, 2016 - royalsocietypublishing.org
To understand basic principles of living organisms one has to know many different
properties of all cellular components, their mutual interactions but also their amounts and …

Imaging live cells at the nanometer-scale with single-molecule microscopy: obstacles and achievements in experiment optimization for microbiology

BL Haas, JS Matson, VJ DiRita, JS Biteen - Molecules, 2014 - mdpi.com
Single-molecule fluorescence microscopy enables biological investigations inside living
cells to achieve millisecond-and nanometer-scale resolution. Although single-molecule …

Fluorescence imaging for bacterial cell biology: from localization to dynamics, from ensembles to single molecules

Z Yao, R Carballido-Lopez - Annual review of microbiology, 2014 - annualreviews.org
Fluorescent proteins and developments in superresolution (nanoscopy) and single-
molecule techniques bring high sensitivity, speed, and one order of magnitude gain in …

ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data

JH Smit, Y Li, EM Warszawik, A Herrmann, T Cordes - PloS one, 2019 - journals.plos.org
Single-molecule fluorescence microscopy studies of bacteria provide unique insights into
the mechanisms of cellular processes and protein machineries in ways that are unrivalled by …

Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis

A Paintdakhi, B Parry, M Campos, I Irnov… - Molecular …, 2016 - Wiley Online Library
With the realization that bacteria display phenotypic variability among cells and exhibit
complex subcellular organization critical for cellular function and behavior, microscopy has …