Multiple-and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: unitary steps and forces
We have developed a new technique for measurements of piconewton forces and
nanometer displacements in the millisecond time range caused by actin-myosin interaction …
nanometer displacements in the millisecond time range caused by actin-myosin interaction …
Single-molecule analysis of the actomyosin motor using nano-manipulation
A Ishijima, Y Harada, H Kojima, T Funatsu… - Biochemical and …, 1994 - Elsevier
The elementary events in energy transduction by the actomyosin motor, driven by ATP
hydrolysis, were directly recorded from multiple and single molecules using a recently …
hydrolysis, were directly recorded from multiple and single molecules using a recently …
Mechanical measurements of single actomyosin motor force.
H Miyata, H Yoshikawa, H Hakozaki, N Suzuki… - Biophysical …, 1995 - ncbi.nlm.nih.gov
To elucidate the mechanism of force generation by actomyosin motor, a measuring system
was constructed, in which an in vitro motility assay was combined with an optical trapping …
was constructed, in which an in vitro motility assay was combined with an optical trapping …
Nano-manipulation of actomyosin molecular motors in vitro: a new working principle
T Yanagida, Y Harada, A Ishijima - Trends in biochemical sciences, 1993 - cell.com
MUSCLE CONTRACTION and other biological motions are propelled by an actomyosin
motor driven by the chemical energy of ATP hydrolysis. Despite numerous studies, the …
motor driven by the chemical energy of ATP hydrolysis. Despite numerous studies, the …
Single molecule analysis of the actomyosin motor
T Yanagida, K Kitamura, H Tanaka, AH Iwane… - Current Opinion in Cell …, 2000 - Elsevier
Progress in imaging techniques and nano-manipulation of single molecules has been
remarkable. These techniques have allowed the accurate determination of myosin-head …
remarkable. These techniques have allowed the accurate determination of myosin-head …
Direct measurement of stiffness of single actin filaments with and without tropomyosin by in vitro nanomanipulation.
H Kojima, A Ishijima… - Proceedings of the …, 1994 - National Acad Sciences
In order to explain the molecular mechanism of muscle contraction, it is crucial to know the
distribution of the sarcomere compliance of active muscle. Here, we directly measure the …
distribution of the sarcomere compliance of active muscle. Here, we directly measure the …
Single–motor mechanics and models of the myosin motor
T Yanagida, S Esaki, AH Iwane… - … of the Royal …, 2000 - royalsocietypublishing.org
Recent progress in single–molecule detection techniques is remarkable. These techniques
have allowed the accurate determination of myosin–head–induced displacements and how …
have allowed the accurate determination of myosin–head–induced displacements and how …
Force generation in single conventional actomyosin complexes under high dynamic load
Y Takagi, EE Homsher, YE Goldman, H Shuman - Biophysical journal, 2006 - cell.com
The mechanical load borne by a molecular motor affects its force, sliding distance, and its
rate of energy transduction. The control of ATPase activity by the mechanical load on a …
rate of energy transduction. The control of ATPase activity by the mechanical load on a …
Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin
A Ishijima, H Kojima, T Funatsu, M Tokunaga… - Cell, 1998 - cell.com
We have developed a technique that allows mechanical and ligand-binding events in a
single myosin molecule to be monitored simultaneously. We describe how steps in the …
single myosin molecule to be monitored simultaneously. We describe how steps in the …
Stepwise Motion of an Actin Filament over a Small Number of Heavy Meromyosin Molecules Is Revealed in an In Vitro Motility Assay
H Miyata, H Hakozaki, H Yoshikawa… - The Journal of …, 1994 - academic.oup.com
In order to determine the relative motions of an actin filament and a myosin molecule upon
hydrolysis of one ATP, an in vitro motility assay, in which individual actin filaments slide over …
hydrolysis of one ATP, an in vitro motility assay, in which individual actin filaments slide over …