Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

S Sahdev, SK Khattar, KS Saini - Molecular and cellular biochemistry, 2008 - Springer
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …

Bacterial systems for production of heterologous proteins

S Zerbs, AM Frank, FR Collart - Methods in enzymology, 2009 - Elsevier
Proteins are the working molecules of all biological systems and participate in a majority of
cellular chemical reactions and biological processes. Knowledge of the properties and …

Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements

J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Advanced technologies for improved expression of recombinant proteins in bacteria: perspectives and applications

SK Gupta, P Shukla - Critical reviews in biotechnology, 2016 - Taylor & Francis
Prokaryotic expression systems are superior in producing valuable recombinant proteins,
enzymes and therapeutic products. Conventional microbial technology is evolving gradually …

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

HP Sørensen, KK Mortensen - Microbial cell factories, 2005 - Springer
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …

[图书][B] Production of recombinant proteins: Novel microbial and eukaryotic expression systems

G Gellissen - 2006 - books.google.com
While the choices of microbial and eukaryotic expression systems for production of
recombinant proteins are many, most researchers in academic and industrial settings do not …

Recombinant protein expression in microbial systems

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
The emergence of recombinant DNA technology during the early 70's set a revolution in
molecular biology. This set of techniques was strengthened even further later on with the …

Towards universal systems for recombinant gene expression

HP Sørensen - Microbial cell factories, 2010 - Springer
Recombinant gene expression is among the most important techniques used both in
molecular and medical research and in industrial settings. Today, two recombinant …

RETRACTED ARTICLE: Strategies for efficient production of heterologous proteins in Escherichia coli

S Jana, JK Deb - Applied microbiology and biotechnology, 2005 - Springer
In recent years, the number of recombinant proteins used for therapeutic applications has
increased dramatically. Production of these proteins has a remarkable demand in the …

Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

MR Dyson, SP Shadbolt, KJ Vincent, RL Perera… - BMC …, 2004 - Springer
Background In the search for generic expression strategies for mammalian protein families
several bacterial expression vectors were examined for their ability to promote high yields of …