Comprehensive comparison of FISH, RT‐PCR, and RQ‐PCR for monitoring the BCR‐ABL gene after hematopoietic stem cell transplantation in CML

YJ Kim, DW Kim, S Lee, HJ Kim, YL Kim… - European journal of …, 2002 - Wiley Online Library
The reverse transcriptase‐polymerase chain reaction (RT‐PCR) was compared with
fluorescence in situ hybridization (FISH) and real‐time quantitative RT‐PCR (RQ‐PCR) for …

[HTML][HTML] Molecular monitoring of BCR-ABL transcripts after allogeneic stem cell transplantation for chronic myeloid leukemia

M Arpinati, G Tolomelli, MT Bochicchio… - Biology of Blood and …, 2013 - Elsevier
The monitoring of minimal residual disease (MRD) through low sensitivity real-time (RT)
polymerase chain reaction (PCR) analysis of BCR-ABL transcripts allows early detection of …

Monitoring of BCR-ABL expression using real-time RT-PCR in CML after bone marrow or peripheral blood stem cell transplantation

M Eder, K Battmer, S Kafert, A Stucki, A Ganser… - Leukemia, 1999 - nature.com
To analyze the value of real time RT-PCR for monitoring of bcr-abl expression in CML
patients after allogeneic or autologous stem cell transplantation (SCT), we generated pairs …

Detection of bcr/abl transcripts by RT-PCR and their colorimetric evaluation in chronic myeloid leukemia patients receiving allogeneic bone marrow transplantation

V Santini, A Zoccolante, A Bosi, S Guidi… - …, 1996 - haematologica.org
BACKGROUND. Chronic myeloid leukemia (CML) is a disease characterized by the
presence of a unique molecular marker, ie the fusion gene bcr-abl and its mRNA and protein …

Early detection of BCR-ABL transcripts by quantitative reverse transcriptase–polymerase chain reaction predicts outcome after allogeneic stem cell transplantation for …

E Olavarria, E Kanfer, R Szydlo, J Kaeda… - Blood, The Journal …, 2001 - ashpublications.org
The reverse transcriptase–polymerase chain reaction (RT-PCR) has become widely used
for monitoring minimal residual disease after allogeneic stem cell transplantation (SCT) for …

Clinical significance of bcr-abl gene rearrangement detected by polymerase chain reaction after allogeneic bone marrow transplantation in chronic myelogenous …

R Delage, RJ Soiffer, K Dear, J Ritz - 1991 - ashpublications.org
In chronic myelogenous leukemia (CML), amplification of a segment of bcr-abl messenger
RNA (mRNA) by polymerase chain reaction (PCR) can be used to detect minimal residual …

Quantitative real‐time reverse‐transcription polymerase chain reaction for diagnosis of BCR‐ABL positive leukemias and molecular monitoring following allogeneic …

F Neumann, C Herold, B Hildebrandt… - European journal of …, 2003 - Wiley Online Library
Real‐time reverse‐transcription polymerase chain reaction (RT‐PCR)(qPCR) of the BCR‐
ABL mRNA is a suitable technique to measure the amount of circulating leukemic cells in …

Simple competitive two-step RT-PCR assay to monitor minimal residual disease in CML patients after bone marrow transplantation

J Moravcova, M Lukášová, J Starý, C Haškovec - Leukemia, 1998 - nature.com
Quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assessing the
amount of transcripts of the BCR/ABL gene, the molecular marker of chronic myeloid …

Polymerase chain reaction detection of the BCR-ABL fusion transcript after allogeneic marrow transplantation for chronic myeloid leukemia: results and implications in …

JP Radich, G Gehly, T Gooley, E Bryant, RA Clift… - 1995 - ashpublications.org
We studied 346 patients after bone marrow transplantation (BMT) for chronic myeloid
leukemia (CML) for the presence of the bcr-abl transcript detected by the polymerase chain …

Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using …

P Bolufer, GF Sanz, E Barragán, MA Sanz… - …, 2000 - haematologica.org
BACKGROUND AND OBJECTIVES: The limited value of qualitative reverse transcription
polymerase chain-reaction (RT-PCR) for monitoring chronic myeloid leukemia (CML) …