Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue–derived stromal and vascular cells

A Trivisonno, G Di Rocco, C Cannistra… - Aesthetic Surgery …, 2014 - academic.oup.com
Background: Adipose tissue is a source of stromal and vascular cells suitable for
regenerative medical applications. Cell recovery depends on several factors, including the …

Naturally adipose stromal cell-enriched fat graft: comparative polychromatic flow cytometry study of fat harvested by barbed or blunt multihole cannula

A Caggiati, A Germani, A Di Carlo… - Aesthetic surgery …, 2017 - academic.oup.com
Background: Fat grafts enriched with cells of the stromal vascular fraction (SVF), especially
adipose-derived stromal cells (ASCs), exhibit significantly improved retention over non …

Harvesting technique affects adipose-derived stem cell yield

T Iyyanki, J Hubenak, J Liu, EI Chang… - Aesthetic Surgery …, 2015 - academic.oup.com
Background The success of an autologous fat graft depends in part on its total stromal
vascular fraction (SVF) and adipose-derived stem cells (ASCs). However, variations in the …

Conventional vs. micro-fat harvesting: how fat harvesting technique affects tissue-engineering approaches using adipose tissue-derived stem/stromal cells

Z Alharbi, C Opländer, S Almakadi, A Fritz… - Journal of Plastic …, 2013 - Elsevier
Background Biocompatible scaffolds as dermal substitutes are used commonly in soft tissue
reconstruction and tissue-engineering approaches. The combination of these scaffolds with …

Use of freshly isolated human adipose stromal cells for clinical applications

RA Lockhart, JA Aronowitz… - Aesthetic Surgery …, 2017 - academic.oup.com
The clinical use of adipose-derived cells is being explored very actively around the world for
various human diseases. Adipose tissue is an abundant tissue source that can be easily …

A standardized method of isolating adipose-derived stem cells for clinical applications

E Raposio, G Caruana, M Petrella… - Annals of Plastic …, 2016 - journals.lww.com
White adipose tissue is the most abundant and accessible source of stem cells in the adult
human body. In this paper, we present a standardised and safe method of isolating and …

Content of soluble factors and characteristics of stromal vascular fraction cells in lipoaspirates from different subcutaneous adipose tissue depots

J Grasys, BS Kim, N Pallua - Aesthetic Surgery Journal, 2016 - academic.oup.com
Background Although fat grafting has emerged as a major force in plastic, reconstructive,
and aesthetic surgery, some questions regarding its reliability and regenerative potential …

Effect of suction pressures on cell yield and functionality of the adipose-derived stromal vascular fraction

YW Chen, JR Wang, X Liao, SH Li, LL Xiao… - Journal of Plastic …, 2017 - Elsevier
Objective The present study was performed to explore the effect of suction pressures on the
cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose …

Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation

A Condé-Green, LS Baptista… - Aesthetic Surgery …, 2010 - academic.oup.com
Background: Centrifugation is one of the preferred methods of fat processing. Although it has
been promoted for nearly three decades to separate adipose tissue components before …

Characterization of structure and cellular components of aspirated and excised adipose tissue

H Eto, H Suga, D Matsumoto, K Inoue… - Plastic and …, 2009 - journals.lww.com
Background: Adipose tissue is an easily accessible tissue for use as a soft-tissue filler and
source of adult multipotent cells, called adipose-derived stem/stromal/progenitor cells …