The vascularization of engineered tissues and organoids has remained a major unresolved
challenge in regenerative medicine. While multiple approaches have been developed to …

The vasculature is an essential component of the circulatory system that plays a vital role in
the development, homeostasis, and disease of various organs in the human body. The …

Sacrificial templates for patterning perfusable vascular networks in engineered tissues have
been constrained in architectural complexity, owing to the limitations of extrusion-based 3D …

Developing methods that provide adequate vascular perfusion is an important step toward
engineering large functional tissues. Meanwhile, an imaging modality to assess the three …

Living systems face a fundamental challenge of orchestrating exchange of nutrients and
oxygen throughout 3D space in order to satisfy their metabolic needs.[1] In nature, vascular …

Living tissues rely heavily on vascular networks to transport nutrients, oxygen and metabolic
waste. However, there still remains a need for a simple and efficient approach to engineer …

This protocol describes how to form a 3D cell culture with explicit, endothelialized
microvessels. The approach leads to fully enclosed, perfusable vessels in a bioremodelable …

A photodegradable material‐based approach to generate endothelialized 3D vascular
networks within cell‐laden hydrogel biomaterials is introduced. Exploiting multiphoton …

One of the pivotal factors that limits the clinical applications of tissue engineering is the
inability to create complex three-dimensional (3D) tissues due to the lack of a long-range …

As engineered tissues progress toward therapeutically relevant length scales and cell
densities, it is critical to deliver oxygen and nutrients throughout the tissue volume via …