Iodine‐enhanced micro‐CT imaging: Methodological refinements for the study of the soft‐tissue anatomy of post‐embryonic vertebrates

PM Gignac, NJ Kley - Journal of Experimental Zoology Part B …, 2014 - Wiley Online Library
The now widespread use of non‐destructive X‐ray computed tomography (CT) and micro‐
CT (µCT) has greatly augmented our ability to comprehensively detail and quantify the …

MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues

BD Metscher - BMC physiology, 2009 - Springer
Background Comparative, functional, and developmental studies of animal morphology
require accurate visualization of three-dimensional structures, but few widely applicable …

Diffusible iodine‐based contrast‐enhanced computed tomography (diceCT): an emerging tool for rapid, high‐resolution, 3‐D imaging of metazoan soft tissues

PM Gignac, NJ Kley, JA Clarke, MW Colbert… - Journal of …, 2016 - Wiley Online Library
Morphologists have historically had to rely on destructive procedures to visualize the three‐
dimensional (3‐D) anatomy of animals. More recently, however, non‐destructive techniques …

[HTML][HTML] The utility of diceCT imaging for high-throughput comparative neuroanatomical studies

PM Gignac, NJ Kley - Brain Behavior and Evolution, 2018 - karger.com
Advancements in imaging techniques have drastically improved our ability to visualize,
study, and digitally share complex, often minute, anatomical relationships. The recent …

MicroCT for developmental biology: A versatile tool for high‐contrast 3D imaging at histological resolutions

BD Metscher - … dynamics: an official publication of the American …, 2009 - Wiley Online Library
Understanding developmental processes requires accurate visualization and
parameterization of three‐dimensional embryos. Tomographic imaging methods offer …

Phosphotungstic acid‐enhanced microCT: Optimized protocols for embryonic and early postnatal mice

KM Lesciotto, SM Motch Perrine… - Developmental …, 2020 - Wiley Online Library
Background Given the need for descriptive and increasingly mechanistic morphological
analyses, contrast‐enhanced microcomputed tomography (microCT) represents perhaps the …

[HTML][HTML] Three-dimensional microCT imaging of mouse development from early post-implantation to early postnatal stages

CW Hsu, L Wong, TL Rasmussen, S Kalaga… - Developmental …, 2016 - Elsevier
In this work, we report the use of iodine-contrast microCT to perform high-throughput 3D
morphological analysis of mouse embryos and neonates between embryonic day 8.5 to …

Small animal computed tomography imaging

SH Bartling, W Stiller, W Semmler… - Current Medical …, 2007 - ingentaconnect.com
Micro Computed Tomography (micro-CT) was suggested in biomedical research to
investigate tissues and small animals. Its use to characterize bone structures, vessels (eg …

Quantitative three‐dimensional imaging of live avian embryonic morphogenesis via micro‐computed tomography

AL Henning, MX Jiang, HC Yalcin… - Developmental …, 2011 - Wiley Online Library
Many clinically relevant congenital malformations arise during mid to late embryonic stages.
This period is challenging to image quantitatively in live embryos, necessitating the use of …

X-ray microtomographic imaging of intact vertebrate embryos

BD Metscher - Cold Spring Harbor Protocols, 2011 - cshprotocols.cshlp.org
X-ray microtomography (microCT) is a highly versatile imaging method, and with appropriate
contrast stains and sample mounting, it can produce high-quality images of embryos at a …