Mining environmental plasmids for synthetic biology parts and devices

E Martínez-García, I Benedetti, A Hueso… - Microbiology …, 2015 - Am Soc Microbiol
The scientific and technical ambition of contemporary synthetic biology is the engineering of
biological objects with a degree of predictability comparable to those made through electric …

[HTML][HTML] The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back

WE Huang, PI Nikel - Microbial Biotechnology, 2019 - ncbi.nlm.nih.gov
The core tenet of synthetic biology is the application of engineering principles such as
standardization, modularity and rational design to accelerate the design-buildtest loop for …

A standardized inverter package borne by broad host range plasmids for genetic circuit design in Gram-negative bacteria

H Tas, Á Goñi-Moreno, V Lorenzo - ACS synthetic biology, 2020 - ACS Publications
Genetically encoded logic gates, especially inverters—NOT gates—are the building blocks
for designing circuits, engineering biosensors, or decision-making devices in synthetic …

The art of vector engineering: towards the construction of next‐generation genetic tools

LC Nora, CA Westmann… - Microbial …, 2019 - Wiley Online Library
When recombinant DNA technology was developed more than 40 years ago, no one could
have imagined the impact it would have on both society and the scientific community. In the …

Unknown unknowns: essential genes in quest for function

A Danchin, G Fang - Microbial Biotechnology, 2016 - Wiley Online Library
The experimental design of a minimal synthetic genome revealed the presence of a large
number of genes without ascribed function, in part because the abstract laws of life must be …

Recent advances in plasmid-based tools for establishing novel microbial chassis

LC Nora, CA Westmann, ME Guazzaroni… - Biotechnology …, 2019 - Elsevier
A key challenge for domesticating alternative cultivable microorganisms with
biotechnological potential lies in the development of innovative technologies. Within this …

GeneGuard: a modular plasmid system designed for biosafety

O Wright, M Delmans, GB Stan, T Ellis - ACS synthetic biology, 2015 - ACS Publications
Synthetic biology applications in biosensing, bioremediation, and biomining envision the
use of engineered microbes beyond a contained laboratory. Deployment of such microbes …

Engineered microbes for therapeutic applications

J Hasty - ACS synthetic biology, 2012 - ACS Publications
As synthetic biologists, we share a focus on the construction of new genetic elements or
circuitry in living cells, whether at the level of large-scale synthesis efforts or the forward …

[HTML][HTML] Synthetic biology: biology by design

MAJ Roberts, RM Cranenburgh, MP Stevens… - Microbiology, 2013 - ncbi.nlm.nih.gov
Synthetic biology can be defined as the design and construction of novel biologically based
parts, devices and systems, as well as the redesign of existing natural biological systems, for …

[HTML][HTML] Synthetic constructs in/for the environment: managing the interplay between natural and engineered Biology

M Schmidt, V de Lorenzo - FEBS letters, 2012 - Elsevier
The plausible release of deeply engineered or even entirely synthetic/artificial
microorganisms raises the issue of their intentional (eg bioremediation) or accidental …