CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
The bacterial origins of the CRISPR genome-editing revolution
EJ Sontheimer, R Barrangou - Human gene therapy, 2015 - liebertpub.com
Like most of the tools that enable modern life science research, the recent genome-editing
revolution has its biological roots in the world of bacteria and archaea. C lustered, r egularly …
revolution has its biological roots in the world of bacteria and archaea. C lustered, r egularly …
Current trends in gene recovery mediated by the CRISPR-Cas system
The CRISPR-Cas system has undoubtedly revolutionized the genome editing field, enabling
targeted gene disruption, regulation, and recovery in a guide RNA-specific manner. In this …
targeted gene disruption, regulation, and recovery in a guide RNA-specific manner. In this …
Target-specific precision of CRISPR-mediated genome editing
AM Chakrabarti, T Henser-Brownhill, J Monserrat… - Molecular cell, 2019 - cell.com
The CRISPR-Cas9 system has successfully been adapted to edit the genome of various
organisms. However, our ability to predict the editing outcome at specific sites is limited …
organisms. However, our ability to predict the editing outcome at specific sites is limited …
The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Global detection of DNA repair outcomes induced by CRISPR–Cas9
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
CRISPR-based technologies for the manipulation of eukaryotic genomes
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …
advances in the life sciences that have grown from the ability to edit genomes within living …
[HTML][HTML] Methods and applications of CRISPR-mediated base editing in eukaryotic genomes
The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …