The magnitude of shigellosis in developing countries is largely unknown because an
affordable detection method is not available. Current laboratory diagnosis of Shigella spp. is …

Background: Shigella species are among the common causes of bacterial diarrhoeal
diseases. Traditional detection methods are time-consuming resulting in delay in treatment …

Abstract Background In Malaysia, Shigella spp. was reported to be the third commonest
bacterial agent responsible for childhood diarrhoea. Currently, isolation of the bacterium and …

In the detection of Shigella species using molecular biological methods, previously known
genetic markers for Shigella species were not sufficient to discriminate between Shigella …

Analysis of single nucleotide polymorphisms (SNPs) is an important genetic tool that
provides molecular markers for rapid differentiation of closely related strains. We have …

Aims: This study was designed to isolate Shigella spp. strains from food and stool samples
by a combination of PCR and culture methods and characterize their serotypes, antibiotic …

The sensitivity and specificity of the polymerase chain reaction (PCR) for detection of DNA
sequences specific to Shigella spp. and enteroinvasive Escherichia coli (EIEC) in stools was …

Shigella spp are exquisitely fastidious Gram negative organisms that frequently get missed
in the detection by traditional culture methods. For this reason, this work has adapted a …

Shigella spp. are exquisitely fastidious gram-negative organisms which frequently escape
detection by traditional culture methods. To get a more complete understanding of the …

Most of the multiplex PCR (mPCR) used to identify Shigella do not discriminate between
Shigella species or serotypes. We designed a mPCR to differentiate between S. flexneri and …