Transcription factors and their target promoters are central to synthetic biology. By arranging
these components into novel gene regulatory circuits, synthetic biologists have been able to …

One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory
components to elicit desired outputs. Gene expression driven by ligand-inducible …

Just like in many engineering systems, impedance-like effects, called retroactivity, arise at
the interconnection of biomolecular circuits, leading to unexpected changes in a circuit's …

Accurate characterization of promoter behavior is essential for the rational design of
functional synthetic transcription networks such as logic gates and oscillators. However …

The survival of cells and organisms requires proper responses to environmental signals.
These responses are governed by cellular networks, which serve to process diverse …

RNA regulators are emerging as powerful tools to engineer synthetic genetic networks or
rewire existing ones. A potential strength of RNA networks is that they may be able to …

Constructing genetic logic circuits is an application of synthetic biology in which parts of the
DNA of a living cell are engineered to perform a dedicated Boolean function triggered by an …

Cell-free genetic circuit elements were constructed in a transcription–translation extract. We
engineered transcriptional activation and repression cascades, in which the protein product …

Synthetic gene circuits perturb the physiology of their cellular host. The extra load on
endogenous processes shifts the equilibrium of resource allocation in the host, leading to …

Synthetic biology promises to revolutionize biotechnology by providing the means to
reengineer and reprogram cellular regulatory mechanisms. However, synthetic gene circuits …