[PDF][PDF] A genetic map of the response to DNA damage in human cells
M Olivieri, T Cho, A Álvarez-Quilón, K Li… - Cell, 2020 - cell.com
The response to DNA damage is critical for cellular homeostasis, tumor suppression,
immunity, and gametogenesis. In order to provide an unbiased and global view of the DNA …
immunity, and gametogenesis. In order to provide an unbiased and global view of the DNA …
FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways
DNA damage-activated signaling pathways are critical for coordinating multiple cellular
processes, which must be tightly regulated to maintain genome stability. To provide a …
processes, which must be tightly regulated to maintain genome stability. To provide a …
Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens
SW Awwad, A Serrano-Benitez, JC Thomas… - … Reviews Molecular Cell …, 2023 - nature.com
All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …
damage response (DDR) processes. Owing to their relevance to human disease, most …
[PDF][PDF] Functional interrogation of DNA damage response variants with base editing screens
Mutations in DNA damage response (DDR) genes endanger genome integrity and
predispose to cancer and genetic disorders. Here, using CRISPR-dependent cytosine base …
predispose to cancer and genetic disorders. Here, using CRISPR-dependent cytosine base …
DNA damage: from threat to treatment
A Carusillo, C Mussolino - Cells, 2020 - mdpi.com
DNA is the source of genetic information, and preserving its integrity is essential in order to
sustain life. The genome is continuously threatened by different types of DNA lesions, such …
sustain life. The genome is continuously threatened by different types of DNA lesions, such …
A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage
Mutational signatures are imprints of pathophysiological processes arising through
tumorigenesis. We generated isogenic CRISPR–Cas9 knockouts (∆) of 43 genes in human …
tumorigenesis. We generated isogenic CRISPR–Cas9 knockouts (∆) of 43 genes in human …
A limited number of double-strand DNA breaks is sufficient to delay cell cycle progression
J van den Berg, A G. Manjón, K Kielbassa… - Nucleic acids …, 2018 - academic.oup.com
DNA damaging agents cause a variety of lesions, of which DNA double-strand breaks
(DSBs) are the most genotoxic. Unbiased approaches aimed at investigating the …
(DSBs) are the most genotoxic. Unbiased approaches aimed at investigating the …
[PDF][PDF] Dissection of DNA damage responses using multiconditional genetic interaction maps
To protect the genome, cells have evolved a diverse set of pathways designed to sense,
signal, and repair multiple types of DNA damage. To assess the degree of coordination and …
signal, and repair multiple types of DNA damage. To assess the degree of coordination and …
A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these
lesions using either end protection-or end resection-coupled mechanisms. To study DSB …
lesions using either end protection-or end resection-coupled mechanisms. To study DSB …
Genetic screens in isogenic mammalian cell lines without single cell cloning
Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools
for understanding gene function. Generating such pairs of mammalian cells, however, is …
for understanding gene function. Generating such pairs of mammalian cells, however, is …