[HTML][HTML] Enrichment of thawed boar spermatozoa with an intact membrane using Magnetic Activated Cell Sorting

A Johannisson, JM Morrell, M Wallgren - Animal Reproduction Science, 2024 - Elsevier
Not all boar sperm samples survive cryopreservation well. A method of eliminating damaged
sperm might enable more cryopreserved boar semen to be used for pig breeding. In this …

[HTML][HTML] Comparison of single layer centrifugation and magnetic activated cell sorting for selecting viable boar spermatozoa after thawing

S Deori, T Ntallaris, M Wallgren, JM Morrell… - Livestock Science, 2022 - Elsevier
Sperm selection techniques, such as magnetic activated cell sorting (MACS) and colloid
centrifugation, are reported to select good quality spermatozoa from semen samples of …

Comparative viability of bovine sperm frozen on a cryomicroscope or in straws

LM Penfold, DL Garner, AM Donoghue, LA Johnson - Theriogenology, 1997 - Elsevier
The accuracy and repeatability of freezing rates and effects of evaporation were examined
using a new cryomicroscope system to establish its usefulness in assessing the …

The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status

SSM Faezah, FMY Zuraina, JHF Farah, O Khairul… - Zygote, 2014 - cambridge.org
Cryopreservation is a technique used to preserve cells for long-time storage. It is widely
used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to …

Elimination of apoptotic boar spermatozoa using magnetic activated cell sorting

J Mrkun, T Dolenšek, T Knific, A Pišlar, M Kosec… - Acta Veterinaria …, 2014 - actavet.vfu.cz
One of the features of apoptosis is the externalization of phosphatidylserine which could be
used to remove apoptotic cells from semen preparations. Magnetic-activated cell sorting …

Assessment of the efficacy of Sephadex G-15 filtration of bovine spermatozoa for cryopreservation

A Januskauskas, K Lukoseviciute, S Nagy… - Theriogenology, 2005 - Elsevier
Semen from five dairy AI bulls was split-filtered through a Sephadex G-15 filter and frozen in
a Tris–citric acid buffer egg yolk-based extender. The effect of filtration was studied …

59 EVALUATION OF CRYOPRESERVED CHICKEN SEMEN WITH DIFFERENT CRYOPROTECTANTS

JS Choi, DB Shin, YG Ko, YJ Do… - Reproduction …, 2014 - CSIRO Publishing
The purpose of this study was to evaluate the viability, acrosome integrity, mitochondrial
activity, and fertility of frozen-thawed fowl semen using different cryoprotective agents. The …

Glass wool filtration of bull cryopreserved semen: a rapid and effective method to obtain a high percentage of functional sperm

MM Arzondo, JN Caballero, CI Marín-Briggiler, G Dalvit… - Theriogenology, 2012 - Elsevier
Frozen-thawed bull sperm are widely used in assisted reproductive technologies, but
cryopreservation negatively affects semen quality. Several sperm selection techniques have …

[HTML][HTML] Multicolor flow cytometric analysis of cryopreserved bovine sperm: a tool for the evaluation of bull fertility

K Bucher, E Malama, M Siuda, F Janett… - Journal of dairy science, 2019 - Elsevier
The study aimed at the analysis of the functional status of cryopreserved bovine sperm using
multicolor flow cytometry. The value of sperm functional traits as predictors of bull fertility was …

Equine spermatozoa selection by magnetic activation for use in assisted reproduction

TI Assumpção, R Lançoni, M Foschini… - Journal of Equine …, 2023 - Elsevier
This study aimed to select high-quality spermatozoa by sperm separation by magnetic
activation of the fresh equine semen, compared to density gradient centrifugation and …