The study of growth (of cells, organ-isms or populations) is inherent to all biological
disciplines. The fundamental properties of a biological system or the physiological effects of …

Figure 1 Continuous single-cell quantification with tTt and qTfy.(a) Iterative image analysis
workflow of tTt: graphical overview of raw data for inspection and loading only of relevant …

Genetically encoded Ca2+ indicators allow researchers to quantitatively measure Ca2+
dynamics in a variety of experimental systems. This protocol summarizes the indicators that …

In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and
drug discovery. Using theoretical modeling and experimentation, we show that current …

Several studies across many fields estimate that only around 40% of published findings can
be replicated reliably. Various funders and communities are promoting ways for …

In the post-genomics era, high-throughput techniques for monitoring RNA1 and protein2
levels are well on their way to becoming mainstream tools for monitoring global cell …

Single-molecule methods (8) overcome the latter difficulty. Like an orchestra, a molecular
ensemble needs to be synchronized for the outcome to be comprehensible. In contrast, a …

To the Editor—The cloning and subsequent engineering of fluorescent proteins (FPs) has
revolutionized methods in biological research1, 2. There are now thousands of FPs …

The increasing complexity of readouts for omics analyses goes hand-in-hand with concerns
about the reproducibility of experiments that analyze'big data'1, 2, 3. When analyzing very …

Background The exploration of microarray data and data from other high-throughput projects
for hypothesis generation has become a vital aspect of post-genomic research. For the non …