Native extracellular matrix probes to target patient-and tissue-specific cell–microenvironment interactions by force spectroscopy

H Holuigue, L Nacci, P Di Chiaro, M Chighizola… - Nanoscale, 2023 - pubs.rsc.org
Atomic Force Microscopy (AFM) is successfully used for the quantitative investigation of the
cellular mechanosensing of the microenvironment. To this purpose, several force …

Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy

PK Viji Babu, C Rianna, U Mirastschijski… - Scientific reports, 2019 - nature.com
Extracellular matrix (ECM), as a dynamic component of the tissue, influences cell behavior
and plays an important role in cell mechanics and tissue homeostasis. Reciprocally, this …

Probing micromechanical properties of the extracellular matrix of soft tissues by atomic force microscopy

I Jorba, JJ Uriarte, N Campillo, R Farré… - Journal of cellular …, 2017 - Wiley Online Library
The extracellular matrix (ECM) determines 3D tissue architecture and provides structural
support and chemical and mechanical cues to the cells. Atomic force microscopy (AFM) has …

Insights in cell biomechanics through atomic force microscopy

S Kerdegari, P Canepa, D Odino, R Oropesa-Nuñez… - Materials, 2023 - mdpi.com
We review the advances obtained by using Atomic Force Microscopy (AFM)-based
approaches in the field of cell/tissue mechanics and adhesion, comparing the solutions …

[HTML][HTML] Force sensing on cells and tissues by atomic force microscopy

H Holuigue, E Lorenc, M Chighizola, C Schulte… - Sensors, 2022 - mdpi.com
Biosensors are aimed at detecting tiny physical and chemical stimuli in biological systems.
Physical forces are ubiquitous, being implied in all cellular processes, including cell …

Single-cell force spectroscopy, an emerging tool to quantify cell adhesion to biomaterials

AV Taubenberger, DW Hutmacher… - Tissue Engineering Part …, 2014 - liebertpub.com
Cell adhesion receptors play a central role in sensing and integrating signals provided by
the cellular environment. Thus, understanding adhesive interactions at the cell–biomaterial …

Force spectroscopy of single cells using atomic force microscopy

A Viljoen, M Mathelié-Guinlet, A Ray… - Nature Reviews …, 2021 - nature.com
Physical forces and mechanical properties have critical roles in cellular function, physiology
and disease. Over the past decade, atomic force microscopy (AFM) techniques have …

Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy

J Friedrichs, J Helenius, DJ Muller - Nature Protocols, 2010 - nature.com
Atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) enables the
quantitative study of cell adhesion under physiological conditions. SCFS probes adhesive …

Bidirectional mechanobiology between cells and their local extracellular matrix probed by atomic force microscopy

J Alcaraz, J Otero, I Jorba, D Navajas - Seminars in cell & developmental …, 2018 - Elsevier
There is growing recognition that the mechanical interactions between cells and their local
extracellular matrix (ECM) are central regulators of tissue development, homeostasis, repair …

Nanomechanics of cells and biomaterials studied by atomic force microscopy

JI Kilpatrick, I Revenko, BJ Rodriguez - 2015 - researchrepository.ucd.ie
The behavior and mechanical properties of cells are strongly dependent on the biochemical
and biomechanical properties of their microenvironment. Thus, understanding the …