The refined crystal structures of the recombinant cytochromebreductase fragment of corn
(Zea mays) nitrate reductase, its ADP complex and the active-site mutant Cys242Ser are …

Background In the biological assimilation of nitrate in plants and microorganisms, nitrate is
reduced to ammonium by transfer of eight electrons in a two-step process. The first step of …

Five cysteine residues in the recombinant cytochrome b reductase domain of corn leaf
NADH: nitrate reductase (EC 1.6. 6.1) were modified by site-directed mutagenesis. At least …

Spectroscopic and kinetic studies comparing the behavior of the recombinant cytochrome b
reductase fragment of corn leaf nitrate reductase and a mutant in which cysteine 242 is …

The cytochromebreductase fragment ofNeurospora crassaNADPH: nitrate reductase (EC
1.6. 6.3) was overexpressed inEscherichia coliwith a His-tag for purification after mutation of …

The recombinant NADH-cytochrome c reductase fragment of spinach NADH-nitrate
reductase (EC 1.6. 6.1), consisting of the contiguous heme-containing cytochrome b domain …

The enzyme nitrate reductase, which catalyzes the reduction of nitrate to nitrite, is a multi-
redox center homodimeric protein. Each polypeptide subunit is approximately 100 kDa in …

Higher plant nitrate reductase can be divided into three functional domains representing its
prosthetic groups: 1) flavin; 2) cytochrome b; and 3) Mo-pterin. The flavin domain has been …

We have analyzed four Nicotiana plumbaginifolia null mutants presumably affected in the
heme domain of nitrate reductase. The DNA sequence of this domain has been determined …

Electrostatic properties on the protein surface were examined on the basis of the crystal
structure of NADH‐cytochrome b5 reductase refined to a crystallographic R factor of 0.223 at …