There is a demand to develop 3rd generation biorefineries that integrate energy production
with the production of higher value chemicals from renewable feedstocks. Here, robust and …

Global demand has driven the use of industrial strains of the yeast Saccharomyces
cerevisiae for large-scale production of biofuels and renewable chemicals. However, the …

Background The thermotolerant methylotrophic yeast Ogataea polymorpha has been
regarded as an important organism for basic research and biotechnological applications. It …

Saccharomyces cerevisiae has been for a long time a common model for fundamental
biological studies and a popular biotechnological engineering platform to produce …

Non-conventional yeasts are playing important roles as cell factories for bioproduction of
biofuels, food additives and proteins with outstanding natural characteristics. However, the …

CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we
present an improved version by using only one single plasmid expressing Cas9 and one or …

As biotechnological applications of synthetic biology tools including multiplex genome
engineering are expanding rapidly, the construction of strategically designed yeast cell …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

Scheffersomyces stipitis, renowned for its native xylose‐utilizing capacity, has recently
demonstrated its potential in producing health‐promoting shikimate pathway derivatives …

Abstract Background The yeast Saccharomyces cerevisiae is increasingly used as a cell
factory. However, cell factory construction time is a major obstacle towards using yeast for …