Optimization of blood handling and peripheral blood mononuclear cell cryopreservation of low cell number samples

CM Hope, D Huynh, YY Wong, H Oakey… - International journal of …, 2021 - mdpi.com
Background: Rural/remote blood collection can cause delays in processing, reducing PBMC
number, viability, cell composition and function. To mitigate these impacts, blood was stored …

Effects of blood transportation on human peripheral mononuclear cell yield, phenotype and function: implications for immune cell biobanking

A Posevitz-Fejfár, V Posevitz, CC Gross, U Bhatia… - PLoS …, 2014 - journals.plos.org
Human biospecimen collection, processing and preservation are rapidly emerging subjects
providing essential support to clinical as well as basic researchers. Unlike collection of other …

[HTML][HTML] Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

A Germann, YJ Oh, T Schmidt, U Schön… - Cryobiology, 2013 - Elsevier
The ability to analyze cryopreserved peripheral blood mononuclear cell (PBMC) from
biobanks for antigen-specific immunity is necessary to evaluate response to immune-based …

Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry

B Li, C Yang, G Jia, Y Liu, N Wang, F Yang, R Su… - BMC immunology, 2022 - Springer
Human peripheral blood mononuclear cells (PBMCs) originate from hematopoietic stem
cells in the bone marrow, which mainly includes lymphocytes (T cells, B cells, and natural …

Effect of warming process on the survival of cryopreserved human peripheral blood mononuclear cells

Y Xu, Q Zou, F Gao, D Wang, S Xue, H Lin… - Biopreservation and …, 2021 - liebertpub.com
It is well known that the warming process is a critical step in cell cryopreservation, affecting
the survival rate of the cryopreserved cells. However, there is a lack of understanding and …

Preliminary report: evaluation of storage conditions and cryococktails during peripheral blood mononuclear cell cryopreservation

LM Cosentino, W Corwin, JM Baust… - Cell Preservation …, 2007 - liebertpub.com
The use of cryopreserved peripheral blood mononuclear cells (PBMCs) has proven critical
in both clinical and biomedical applications. While utilized, in numerous settings, the …

[HTML][HTML] Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation

R Nazarpour, E Zabihi, E Alijanpour… - … journal of molecular …, 2012 - ncbi.nlm.nih.gov
Optimization of Human Peripheral Blood Mononuclear Cells (PBMCs) Cryopreservation - PMC
Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

Toward optimal cryopreservation and storage for achievement of high cell recovery and maintenance of cell viability and T cell functionality

S Angel, H von Briesen, YJ Oh, MK Baller… - Biopreservation and …, 2016 - liebertpub.com
Cryopreservation of biological materials such as cells, tissues, and organs is a prevailing
topic of high importance. It is employed not only in many research fields but also in the …

A comparison of cryopreservation methods: Slow-cooling vs. rapid-cooling based on cell viability, oxidative stress, apoptosis, and CD34+ enumeration of human …

T Djuwantono, FF Wirakusumah, TH Achmad… - BMC research …, 2011 - Springer
Background The finding of human umbilical cord blood as one of the most likely sources of
hematopoietic stem cells offers a less invasive alternative for the need of hematopoietic stem …

Quantitative and functional assessment of the influence of routinely used cryopreservation media on mononuclear leukocytes for medical research

P Haider, T Hoberstorfer, M Salzmann… - International Journal of …, 2022 - mdpi.com
Quantitative and functional analysis of mononuclear leukocyte populations is an invaluable
tool to understand the role of the immune system in the pathogenesis of a disease …