CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of
plants. Nevertheless, unintended off-target edits can arise that might confer risks when …

CRISPR/Cas9 has been widely applied to various plant species accelerating the pace of
plant genome editing and precision breeding in crops. Unintended effects beyond off-target …

The CRISPR‐Cas9 system (clustered regularly interspaced short palindromic repeats with
associated Cas9 protein) has been used to generate targeted changes for direct …

The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

CRISPR/Cas-mediated editing has revolutionized crop engineering. Due to the broad scope
and potential of this technology, many studies have been carried out in the past decade …

The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …

The ability to modify complex genomes precisely to create specific mutants is the holy grail
of basic research and applied genetics in the postgenomic era. Programmable sequence …

Increased understanding of plant genetics and the development of powerful and easier-to-
use gene editing tools over the past century have revolutionized humankind's ability to …

Key message We present novel observations of high-specificity SpCas9 variants, sgRNA
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …