Abstract For 3-dimensional (3D) imaging of a tissue, 3 methodological steps are essential
and their successful application depends on specific characteristics of the type of tissue. The …

Three‐dimensional reconstruction of tissue structures is essential for biomedical research.
The development of light microscopes and various fluorescent labeling techniques provides …

The last decade has seen a proliferation of tissue clearing methods that render large
biological samples transparent and allow unprecedented three-dimensional views of …

Tissue clearing has become an important tool for the investigation of biological systems in
three dimensions. However, many pioneering techniques were based on serendipitous …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional
reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing …

Opaqueness of animal tissue can be attributed mostly to light absorption and light scattering.
In most noncleared tissue samples, confocal images can be acquired at no more than a 100 …

Organisms are inherently three dimensional, thus comprehensive understanding of the
complicated biological system requires analysis of organs or even whole bodies in the …

Summary Three-dimensional (3D) optical imaging techniques can expand our knowledge
about physiological and pathological processes that cannot be fully understood with 2D …

Modern clearing techniques for the three-dimensional (3D) visualisation of neural tissue
microstructure have been very effective when used on rodent brain but very few studies …