Culture of vitrified bovine ovarian tissue on agarose gel inserts maintains follicle integrity

IM Alkali, M Colombo, AM Luciano, W Nizanski… - …, 2023 - rep.bioscientifica.com
In brief Ovarian tissue cryopreservation and culture provide an option for fertility preservation
without tissue grafting, but need optimization. This study reveals that vitrified bovine ovarian …

Novel wide-capacity method for vitrification of caprine ovaries: Ovarian Tissue Cryosystem (OTC)

AA Carvalho, LR Faustino, CMG Silva… - Animal Reproduction …, 2013 - Elsevier
In this study we aimed testing the efficiency of a newly developed device for vitrification of
ovaries without contact with liquid nitrogen, Ovarian Tissue Cryosystem (OTC). From each …

Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice

SU Chen, CL Chien, MY Wu, TH Chen… - Human …, 2006 - academic.oup.com
BACKGROUND: Cryopreservation of ovarian tissue is valuable for fertility preservation. We
develop an innovative vitrification method using less concentrated cryoprotectants and direct …

Frozen and Fresh Ovarian Tissue Require Different Culture Media to Promote in Vitro Development of Bovine Preantral Follicles

SV Castro, AA Carvalho, CMG Silva… - Biopreservation and …, 2014 - liebertpub.com
The aim of this study was to evaluate the efficiency of different media in the in vitro culture of
bovine preantral follicles that were used either fresh or following slow freezing treatment …

Fresh and vitrified bovine preantral follicles have different nutritional requirements during in vitro culture

SV Castro, AA Carvalho, CMG Silva, FW Santos… - Cell and tissue …, 2014 - Springer
The aim of this study was to compare the efficiency of different media for the in vitro culturing
of fresh and vitrified bovine ovarian tissues. Fragments of the ovarian cortex were subjected …

Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation

G Fasano, F Moffa, J Dechène, Y Englert… - Reproductive Biology …, 2011 - Springer
Background In the past few years, cryopreservation of ovarian tissue has become an
established procedure proposed in many centers around the world and transplantation has …

Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?

JM Zhang, Y Sheng, YZ Cao, HY Wang… - Journal of assisted …, 2011 - Springer
Purpose To compare the efficacy of vitrification and conventional freezing of whole ovaries.
Methods Ovaries obtained from 5-year-old female bovines were cryopreserved by …

Oocyte retrieval after heterotopic transplantation of ovarian tissue cryopreserved by closed vitrification protocol

Y Sugishita, N Okamoto, A Uekawa, T Yamochi… - Journal of Assisted …, 2018 - Springer
Purpose A device for closed vitrification was designed to reduce the risk of contamination
and investigated on its efficacy for ovarian function recovery after cryopreservation and …

In vitro survival of follicles in prepubertal ewe ovarian cortex cryopreserved by slow freezing or non-equilibrium vitrification

Y Locatelli, L Calais, N Duffard, L Lardic… - Journal of Assisted …, 2019 - Springer
Purpose Vitrification is a well-accepted fertility preservation procedure for cryopreservation
of oocytes and embryos but little is known regarding ovarian tissue, for which slow freezing …

Presence of sucrose in the vitrification solution and exposure for longer periods of time improve post‐warming follicle integrity in cat ovarian tissues

L Mouttham, P Comizzoli - Reproduction in Domestic Animals, 2017 - Wiley Online Library
Contents Ovarian tissue cryopreservation followed by tissue culture is a promising approach
to preserving the fertility of biomedical models and endangered species. The objective of …