In plant tissue culture, callus formation serves as a crucial mechanism for regenerating
entire plants, enabling the differentiation of diverse tissues. Researchers have extensively …

During studies to optimize production of morphogenic callus from cultured leaf discs of
sugarbeet (Beta vulgaris L.) large differences were observed associated with the gelling …

The gel in a solidified tissue culture medium can influence explant or callus growth and
morphology in a concentration-dependent manner. Depending on the type and stage of …

Metabolic changes were studied, which accompanied the conversion of 6month old HiII
maize non-regenerable (NR) calli into regenerable (R) calli when cultured for 63days with …

Gels and mechanical supports have been used in the culture of microorganisms and plants
for nearly 200 years. The early studies to develop methods of growing and examining …

The efficacies of sago (from Metroxylon sagu Rottb.) and isubgol (from Plantago ovata
Forsk.) as gelling agents and those of filter paper, nylon cloth, polystyrene foam and glass …

The effects of Phytagel, Canageenen, Agar A and Agar powder on callus induction and
embryogenic callus development from the mature seeds of Agrostis palustris 'L293', Poa …

A deliberate change in the growth regulators added to a tissue culture medium can elicit a
dramatic response in a cultured plant. This cause-effect relationship is well-recognized, and …

This study attempted to identify low-cost gelling agents in order to replace agar as one of the
most expensive ingredients in the culture media. Therefore, experiments with 17 alternative …

This chapter covers details of initiation of callus culture and different growth conditions and
how to optimize them depending on the objectives of the investigation. Anyone new to tissue …