The Bacillus subtilis endo-β-1, 4-glucanase gene (beg), which encodes the enzyme BEG
that comprises 499 amino acid residues, was mutated by error-prone polymerase chain …

In order to improve some characteristics of a β‐1, 3‐1, 4‐glucanase from Bacillus subtilis
MA139, directed evolution was conducted in this study. After error‐prone PCR, the β‐1, 3‐1 …

In vitro evolution methods are often used to modify protein with improved characteristics. We
developed a directed evolution protocol to enhance the thermostability of the beta-1, 3-1, 4 …

Abstract β-1, 3-1, 4-Glucanase (BGlc8H) from Paenibacillus sp. X4 was mutated by error-
prone PCR or truncated using termination primers to improve its enzyme properties. The …

Abstract Bacillus subtilis endo-β-1, 4-glucanase (Cel5A) hydrolyzes cellulose by cleavage of
the internal bonds in the glucose chains, producing new ends randomly. Using directed …

In order to improve the thermostability of β-1, 3-1, 4-glucanase, evolutionary molecular
engineering was used to evolve the β-1, 3-1, 4-glucanase from Bacillus subtilis ZJF-1A5 …

Abstract The cytoplasmic endo-$\beta $-l, 4-glucanase (endoglucanase) was purified from
cell extracts of Escherichia coli (pBS1) transformant carrying the Bacillus subtilis endo …

BACKGROUND Error‐prone polymerase chain reaction (PCR) is frequently used in directed
evolution of enzymes to modify their quality. In this study, error‐prone PCR was used to …

The endoglucanase, EGA, from Bacillus sp. AC-1 comprises a glycosyl hydrolase family-9
catalytic module (CM9) and a family-3 carbohydrate-binding module (CBM3). Seven …

The C-terminus region of endo-β-glucanase Egl499 from Bacillus subtilis JA18 was
suggested to be a putative family 3 cellulose-binding domain (CBD) by computer analysis …