Utilization of freeze-thawing method for high-level expression of functional human epidermal growth factor (hEGF)

M Mohammadi, S Ghanbari, A Emamgholi… - International Journal of …, 2023 - Springer
The wide applications of human epidermal growth factor (hEGF) as an efficient mitogen
agent in the clinical, health, and cosmetic industries has made it an interesting research …

Expression of human epidermal growth factor using Ssp DnaB mini-intein as fusion partner in Escherichia coli BL21 (DE3)

Y Yosua, R Melati, S Sriwidodo… - Journal of Applied …, 2021 - japsonline.com
The human epidermal growth factor (hEGF) is widely used clinically as a wound healer, as it
has a vital role in stimulating cell proliferation, differentiation, and migration. Consequently …

Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from …

K Sharma, PVC Babu, P Sasidhar, VK Srinivas… - Protein expression and …, 2008 - Elsevier
Amongst the various endogenous growth factors, epidermal growth factor (EGF) plays an
important role in normal wound healing of tissue such as skin, cornea and gastrointestinal …

Expression and purification of human epidermal growth factor (hEGF) fused with GB1

X Zheng, X Wu, X Fu, D Dai, F Wang - Biotechnology & …, 2016 - Taylor & Francis
Fusion expression is a promising strategy for the production of bioactive peptides in
Escherichia coli. In this study, we constructed a new recombinant expression plasmid …

[PDF][PDF] Refolding of bioactive human epidermal growth factor from E. coli BL21 (DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity

IP Maksum, Y Yosua, A Nabiel, RD Pratiwi… - Heliyon, 2022 - cell.com
Human epidermal growth factor (hEGF) is a mitogenic protein widely used in pharmaceutical
and cosmetic industries, thus recombinant DNA technology has been applied to meet the …

An approach for recombinant epidermal growth factor purification using an elastin-like protein tag

R Sarvestani, AM Latifi, H Alizadeh… - Journal of Applied …, 2021 - eprints.bmsu.ac.ir
Introduction: Wide applications in research, clinical and cosmetic industry of human
epidermal growth factor (hEGF) has made it as a research interest target. Its production in …

High-level expression and purification of human epidermal growth factor with SUMO fusion in Escherichia coli

Z Su, Y Huang, Q Zhou, Z Wu, X Wu… - Protein and peptide …, 2006 - ingentaconnect.com
Human epidermal growth factor (hEGF) can stimulate the division of various cell types and
has potential clinical applications. However, the high expression of active hEGF in …

Production of recombinant human epidermal growth factor in Bacillus subtilis

HH Su, JC Chen, PT Chen - Journal of the Taiwan Institute of Chemical …, 2020 - Elsevier
Human epidermal growth factor (hEGF) is a crucial factor in wound healing, and therefore
has wide applications in the pharmaceutical and cosmetics industries. To improve the …

Fusion with pep-1, a cell-penetrating peptide, enhances the transmembrane ability of human epidermal growth factor

XG Luo, DY Ma, Y Wang, W Li, CX Wang… - Bioscience …, 2016 - academic.oup.com
Administration of macromolecule compositions in medicine and cosmetics always exhibited
low bioavailability due to the limitation of transmembrane transport. Here, human epidermal …

[HTML][HTML] High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli

Y Ma, J Yu, J Lin, S Wu, S Li, J Wang - BioMed research international, 2016 - hindawi.com
Human epidermal growth factor (hEGF) is a small, mitotic growth polypeptide that promotes
the proliferation of various cells and is widely applied in clinical practices. However, high …