Improvements in technology have reduced the cost of DNA sequencing to the point that the
limiting factor for many experiments is the time and reagent cost of sample preparation. We …

The large amount of DNA sequence data generated by high-throughput sequencing
technologies often allows multiple samples to be sequenced in parallel on a single …

New sequencing technologies can address diverse biomedical questions but are limited by
a minimum required DNA input of typically 1 μg. We describe how sequencing libraries can …

Although the emergence of high-throughput sequencing technologies has enabled whole-
genome sequencing from extinct organisms, little progress has been made in accelerating …

Fifteen years elapsed between the discovery of the double helix (1953) and the first DNA
sequencing (1968). Modern DNA sequencing began in 1977, with development of the …

Targeted genome enrichment is a powerful tool for making use of the massive throughput of
novel DNA-sequencing instruments. We herein present a simple and scalable protocol for …

Targeted, capture-based DNA sequencing is a cost-effective method to focus sequencing on
a coding region or other customized region of the genome. There are multiple targeted …

High-throughput 454 DNA sequencing technology allows much faster and more cost-
effective sequencing than traditional Sanger sequencing. However, the technology imposes …

Massively parallel DNA sequencing technologies have greatly increased our ability to
generate large amounts of sequencing data at a rapid pace. Several methods have been …

Abstract When the Human Genome Mapping Project began in earnest, the allocation of
large funding and the opening of dedicated research facilities promised much for the rapid …