Reversible phosphorylation is a fundamental mechanism for controlling protein function.
Despite the critical roles phosphorylated proteins play in physiology and disease, our ability …

Genetic Code Expansion (GCE) can use TAG stop codons to guide site-specific
incorporation of phosphoserine (pSer) into proteins. To eliminate prematurely truncated …

Protein phosphorylation is a reversible, residue-specific posttranslational modification that
plays a pivotal role in cell signaling, and the phosphorylation state of proteins is tightly …

Phosphorylation, one of the important protein post-translational modifications, is involved in
many essential cellular processes. Site-specifical and homogeneous phosphoproteins can …

Studies on the mechanism of protein phosphorylation and therapeutic interventions of its
related molecular processes are limited by the difficulty in the production of purpose-built …

Serine phosphorylation is a key post-translational modification that regulates diverse
biological processes. Powerful analytical methods have identified thousands of …

While site-specific translational encoding of phosphoserine (pSer) into proteins in
Escherichia coli via genetic code expansion (GCE) technologies has transformed our ability …

Highlights•Classical site-directed mutagenesis is limited in studying protein
phosphorylation.•We discuss synthetic, semisynthetic, and genetic methods to make …

Understanding the functional and structural consequences of site-specific protein
phosphorylation has remained limited by our inability to produce phosphoproteins at high …

The universal genetic code, which specifies the 20 standard amino acids (AAs), forms the
basis for all natural proteins. Researchers have developed efficient and robust in vivo and in …