DNA extraction procedures meaningfully influence qPCR-based mtDNA copy number determination
Quantitative real time PCR (qPCR) is commonly used to determine cell mitochondrial DNA
(mtDNA) copy number. This technique involves obtaining the ratio of an unknown variable …
(mtDNA) copy number. This technique involves obtaining the ratio of an unknown variable …
PCR-based determination of mitochondrial DNA copy number in multiple species
TC Leuthner, JH Hartman, IT Ryde… - … Regulation: Methods and …, 2021 - Springer
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial
health. In this chapter, we describe methods for simultaneous isolation of mtDNA and …
health. In this chapter, we describe methods for simultaneous isolation of mtDNA and …
PCR based determination of mitochondrial DNA copy number in multiple species
JP Rooney, IT Ryde, LH Sanders, EH Howlett… - … : Methods and Protocols, 2015 - Springer
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial
health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA …
health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA …
Quantification of mitochondrial DNA copy number: pre-analytical factors
AL Andreu, R Martinez, R Marti, E García-Arumí - Mitochondrion, 2009 - Elsevier
Mitochondrial DNA (mtDNA) content is important for understanding many cellular processes.
Several pre-analytical factors, from sample collection to DNA extraction can affect …
Several pre-analytical factors, from sample collection to DNA extraction can affect …
Development of a triplex mtDNA qPCR assay to assess quantification, degradation, inhibition, and amplification target copy numbers
MF Kavlick - Mitochondrion, 2019 - Elsevier
A hybrid absolute/relative qPCR assay which provides information regarding the condition of
mitochondrial DNA (mtDNA) in a DNA sample is described. MtDNA concentration (copy …
mitochondrial DNA (mtDNA) in a DNA sample is described. MtDNA concentration (copy …
Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution
Mitochondria are involved in a number of diverse cellular functions, including energy
production, metabolic regulation, apoptosis, calcium homeostasis, cell proliferation, and …
production, metabolic regulation, apoptosis, calcium homeostasis, cell proliferation, and …
Accurate measurement of circulating mitochondrial DNA content from human blood samples using real-time quantitative PCR
S Ajaz, A Czajka, A Malik - Mitochondrial Medicine: Volume I, Probing …, 2015 - Springer
We describe a protocol to accurately measure the amount of human mitochondrial DNA
(MtDNA) in peripheral blood samples which can be modified to quantify MtDNA from other …
(MtDNA) in peripheral blood samples which can be modified to quantify MtDNA from other …
Mitochondrial DNA as a non-invasive biomarker: accurate quantification using real time quantitative PCR without co-amplification of pseudogenes and dilution bias
AN Malik, R Shahni, A Rodriguez-de-Ledesma… - Biochemical and …, 2011 - Elsevier
Circulating mitochondrial DNA (MtDNA) is a potential non-invasive biomarker of cellular
mitochondrial dysfunction, the latter known to be central to a wide range of human diseases …
mitochondrial dysfunction, the latter known to be central to a wide range of human diseases …
Evaluation of mitochondrial DNA copy number estimation techniques
Mitochondrial DNA copy number (mtDNA-CN), a measure of the number of mitochondrial
genomes per cell, is a minimally invasive proxy measure for mitochondrial function and has …
genomes per cell, is a minimally invasive proxy measure for mitochondrial function and has …
[HTML][HTML] Quantification of mitochondrial DNA copy number in suspected cancer patients by a well optimized ddPCR method
AA Memon, B Zöller, A Hedelius, X Wang… - Biomolecular detection …, 2017 - Elsevier
Abstract Changes in mitochondrial DNA (mtDNA) content is a useful clinical biomarker for
various diseases, however results are controversial as several analytical factors can affect …
various diseases, however results are controversial as several analytical factors can affect …