[HTML][HTML] Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

FM Dupont, WH Vensel, CK Tanaka, WJ Hurkman… - Proteome Science, 2011 - Springer
Background Wheat flour is one of the world's major food ingredients, in part because of the
unique end-use qualities conferred by the abundant glutamine-and proline-rich gluten …

One hundred years of grain omics: identifying the glutens that feed the world

M Ribeiro, JD Nunes-Miranda, G Branlard… - Journal of Proteome …, 2013 - ACS Publications
Glutens, the storage proteins in wheat grains, are a major source of protein in human
nutrition. The protein composition of wheat has therefore been an important focus of cereal …

Proteomic profiling of 16 cereal grains and the application of targeted proteomics to detect wheat contamination

ML Colgrave, H Goswami, K Byrne… - Journal of proteome …, 2015 - ACS Publications
Global proteomic analysis utilizing SDS-PAGE, Western blotting and LC–MS/MS of total
protein and gluten-enriched extracts derived from 16 economically important cereals was …

Wheat cultivar‐specific proteins in grain revealed by 2‐DE and their application to cultivar identification of flour

E Yahata, W Maruyama‐Funatsuki, Z Nishio… - …, 2005 - Wiley Online Library
Wheat flour proteins were studied to identify the cultivar‐specific proteins and use them to
identify cultivars in flours. Proteins extracted from flours of Japanese wheat (cultivars …

[HTML][HTML] Comprehensive proteomic profiling of wheat gluten using a combination of data-independent and data-dependent acquisition

SNL Bromilow, LA Gethings, JI Langridge… - Frontiers in plant …, 2017 - frontiersin.org
Wheat is the most important food crop in the world, the unique physiochemical properties of
wheat gluten enabling a diverse range of food products to be manufactured. However …

[HTML][HTML] Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

G Guo, D Lv, X Yan, S Subburaj, P Ge, X Li, Y Hu… - BMC plant biology, 2012 - Springer
Background The analyses of protein synthesis, accumulation and regulation during grain
development in wheat are more complex because of its larger genome size compared to …

Optimisation of protein extraction for in-depth profiling of the cereal grain proteome

U Bose, JA Broadbent, K Byrne, S Hasan, CA Howitt… - Journal of …, 2019 - Elsevier
Cereal grain proteomics can provide valuable information regarding plant growth, nutritional
status, adaptation to environmental stresses, and the role that grain proteins play in health …

Characterization of wheat gluten proteins by HPLC and MALDI TOF mass spectrometry

Y Qian, K Preston, O Krokhin, J Mellish… - Journal of the American …, 2008 - Springer
We have performed a detailed characterization and identification of wheat gluten proteins
obtained from the Teal variety of Canadian hard red spring wheat. RP-HPLC separation of …

Proteomic‐based analytical approach for the characterization of glutenin subunits in durum wheat

G Mamone, SD Caro, AD Luccia… - Journal of Mass …, 2009 - Wiley Online Library
One of the main objectives of wheat glutenin subunit (GS) analysis is the identification of
protein components linked to wheat quality. The proteomic characterization of glutenin has …

Characterization of grain-specific peptide markers for the detection of gluten by mass spectrometry

KL Fiedler, SC McGrath, JH Callahan… - Journal of agricultural …, 2014 - ACS Publications
Global and targeted mass spectrometry-based proteomic approaches were developed to
discover, evaluate, and apply gluten peptide markers to detect low parts per million (ppm) …