Molecular methods may reveal the presence of pathogens in samples through the detection
of specific target gene (s) associated with microorganisms, but often, the subsequent cultural …

Detection of Shiga toxin-producing Escherichia coli (STEC) by culture methods is advisable
to identify the pathogen, but recovery of the strain responsible for the disease is not always …

Different experimental approaches were evaluated for their ability to detect stx genes by
PCR and identify Shiga toxin-producing Escherichia coli (STEC) in bovine fecal samples …

Materials and Methods Four hundred and twenty samples consisted of recto-anal mucosal
swabs were collected from cattle. They were checked for the presence of the stx1 and stx2 …

The dynamics related to the loss of stx genes from Shiga toxin-producing Escherichia coli
remain unclear. Current diagnostic procedures have shortcomings in the detection and …

Sheep faeces may be an important source of Shiga toxin (Stx)-producing Escherichia coli.
We have, therefore, established and evaluated a real-time 5′-nuclease PCR assay to …

Aims: To investigate if cattle on the same farm as sheep are a possible risk factor for stx in
sheep and to determine whether or not sheep and cattle on the same farm share the same …

Today, it is nearly 25 years past from investigation of Shiga toxigenic Escherichia coli
(STEC) which is able to produce Shiga toxins and cause different gastroenteritis. Since …

Pets can be reservoirs of Shiga toxin-producing Escherichia coli (STEC) strains. The aim of
this study was to examine nine strains belonging to several serotypes (O91: H21, O91: H16 …

Shiga toxin–producing Escherichia coli (STEC) are major foodborne human pathogens that
cause mild to hemorrhagic colitis, which could lead to complications of hemolytic uremic …