Genome editing continues to revolutionize biological research. Due to its simplicity and
flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems …

Transcription-activator-like effector (TALE)-based tools for base editing of nuclear and
organellar DNA rely on double-stranded DNA deaminases, which edit substrate bases on …

Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …

Chloroplast DNA (cpDNA) encodes up to 315 (typically, 120–130) genes, including those for
essential components in photosystems I and II and the large subunit of RuBisCo, which …

Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically
impaired CRISPR–Cas9 variant, are powerful new tools for targeted base editing in cells …

Gene-editing systems have emerged as bioengineering tools in recent years. Classical
gene-editing systems include zinc-finger nucleases (ZFNs), transcription activator-like …

CRISPR/Cas-derived base editing tools empower efficient alteration of genomic cytosines or
adenines associated with essential genetic traits in plants and animals. Diversified target …

CRISPR‐Cas9 has proven to be highly valuable for genome editing in plants, including the
model plant Physcomitrium patens. However, the fact that most of the editing events …

Targeted genome‐editing technology using designed nucleases has been evolving rapidly,
and its applications are widely expanding in research, medicine and biotechnology. Using …

Genome‐editing technologies consisting of targeted mutagenesis and gene targeting
enable us to modify genes of interest rapidly and precisely. The discovery in 2012 of …