Forensic implications of PCR inhibition—a review

R Alaeddini - Forensic Science International: Genetics, 2012 - Elsevier
Polymerase chain reaction (PCR) is currently the method of choice for the identification of
human remains in forensic coursework. DNA samples from crime scenes often contain co …

A comparison of four methods for PCR inhibitor removal

Q Hu, Y Liu, S Yi, D Huang - Forensic science international: Genetics, 2015 - Elsevier
Biological samples collected from the crime scenes often contain some compounds that can
inhibit the polymerase chain reaction (PCR). The removal of PCR inhibitors from the extracts …

A Study of PCR Inhibition Mechanisms Using Real Time PCR*,

KL Opel, D Chung, BR McCord - Journal of forensic sciences, 2010 - Wiley Online Library
In this project, real time polymerase chain reaction (PCR) was utilized to study the
mechanism of PCR inhibition through examination of the effect of amplicon length, melting …

Evaluation of real‐time PCR amplification efficiencies to detect PCR inhibitors

EJ Kontanis, FA Reed - Journal of forensic sciences, 2006 - Wiley Online Library
Real‐time PCR analysis is a sensitive template DNA quantitation strategy that has recently
gained considerable attention in the forensic community. However, the utility of real‐time …

[PDF][PDF] An introduction to PCR inhibitors

J Bessetti - J Microbiol Methods, 2007 - promega.com
For as long as scientists have used the polymerase chain reaction (PCR), PCR inhibitors
have been an obstacle to success. All who use PCR are likely to be impacted by inhibitors at …

NaOH treatment to neutralize inhibitors of Taq polymerase

MT Bourke, CA Scherczinger… - Journal of …, 1999 - asmedigitalcollection.asme.org
The introduction of polymerase chain reaction (PCR) into the forensic field has greatly
extended the ability to analyze DNA from small or degraded samples. However, one …

A quantitative PCR assay for the assessment of DNA degradation in forensic samples

KL Swango, MD Timken, MD Chong… - Forensic science …, 2006 - Elsevier
A multiplex quantitative PCR assay has been designed to amplify target sequences of
different length, which allows for the assessment of DNA degradation in samples of forensic …

[PDF][PDF] Assessing PCR inhibition from humic substances

CD Matheson, C Gurney, N Esau… - The Open Enzyme …, 2010 - researchgate.net
Inhibition remains the greatest methodological challenge in molecular analysis of buried
biological remains. Inhibitory compounds associated with soil environments comprise …

Forensic evaluation of the QIAshredder/QIAamp DNA extraction procedure

V Castella, N Dimo-Simonin… - Forensic science …, 2006 - Elsevier
The potential to recover genetic profiles from evidence samples has substantially increased
since robust and sensitive amplification kits are commercially available. Nevertheless, even …

[HTML][HTML] Impact of metal ions on PCR inhibition and RT-PCR efficiency

A Kuffel, A Gray, NN Daeid - International journal of legal medicine, 2021 - Springer
Inhibition of PCR by metal ions can pose a serious challenge in the process of forensic DNA
analysis. Samples contaminated with various types of metal ions encountered at crime …