Reactivity of urinary albumin (microalbumin) assays with fragmented or modified albumin

D Sviridov, SK Drake, GL Hortin - Clinical chemistry, 2008 - academic.oup.com
Background: Controversy exists regarding occurrence and measurement of structural
variants of albumin in urine. In this study, we examined cross-reactivity of in vitro modified …

Immunochemically unreactive albumin in urine: fiction or reality?

MM Speeckaert, R Speeckaert… - Critical reviews in …, 2011 - Taylor & Francis
Urinary albumin measurements are currently not standardized due to a lack of a reference
method and reference (primary and secondary [matrix]) material. Multiple molecular forms of …

Differences in urinary albumin detected by four immunoassays and high-performance liquid chromatography

WD Comper, G Jerums, TM Osicka - Clinical biochemistry, 2004 - Elsevier
Objectives: To compare the analysis of urinary albumin from diabetic patients by four
conventional immunoassays including radioimmunoassay (RIA), immunonephelometry (IN) …

Characterization of immunochemically nonreactive urinary albumin

TM Osicka, WD Comper - Clinical chemistry, 2004 - academic.oup.com
Background: Conventional immunoassays underestimate the urinary albumin concentration
because intact albumin in urine exists in two forms, immunoreactive and immunochemically …

Comparison of urinary albumin quantification by immunoturbidimetry, competitive immunoassay, and protein-cleavage liquid chromatography–tandem mass …

JC Seegmiller, D Sviridov, TS Larson… - Clinical …, 2009 - academic.oup.com
Background: Increased urinary albumin excretion is a well-documented diagnostic and
prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical …

Preparation of highly purified monomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays

Y Itoh, K Ichihara, K Kishi, S Hosogaya, T Yamada - Clinica Chimica Acta, 2012 - Elsevier
BACKGROUND: International external quality assessments have shown variation in results
of urinary albumin among various immunoassays. A well-defined candidate reference …

Variability of standard clinical protein assays in the analysis of a model urine solution of fragmented albumin

GA Eppel, S Nagy, MA Jenkins, RN Tudball… - Clinical …, 2000 - Elsevier
Objectives: This study investigates the sensitivity of various standard clinical techniques in
the detection of albumin fragments. The significance of this work is in the detection of urinary …

The analysis and characterisation of immuno-unreactive urinary albumin in healthy volunteers

SP Clavant, SA Sastra, TM Osicka, WD Comper - Clinical biochemistry, 2006 - Elsevier
OBJECTIVES:: To compare the analysis of different forms of intact albumin in urine from
healthy volunteers. To determine contamination by common non-albumin proteins on HPLC …

A rapid and sensitive immunosorbent assay for urinary albumin

GJ Van Kamp, JSM Van Bezu… - Annals of clinical …, 1989 - journals.sagepub.com
We describe a fast, simple and sensitive microliter scale competitive ELISA for the
determination of urinary albumin. All reagents are commercially available. In the standard …

On-site quantification of human urinary albumin by a fluorescence immunoassay

S Choi, EY Choi, HS Kim, SW Oh - Clinical chemistry, 2004 - academic.oup.com
Microalbuminuria (MAU), defined as a urinary albumin excretion of 30–300 mg/day,
indicates a high probability of renal damage and is an accepted predictor for the early …