Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches

K Hadjidemetriou, S Kaur, CK Cassidy… - Biochemical Society …, 2022 - portlandpress.com
Chemotaxis signaling pathways enable bacteria to sense and respond to their chemical
environment and, in some species, are critical for lifestyle processes such as biofilm …

Studying bacterial chemosensory array with CryoEM

Z Qin, P Zhang - Biochemical Society Transactions, 2021 - portlandpress.com
Bacteria direct their movement in respond to gradients of nutrients and other stimuli in the
environment through the chemosensory system. The behavior is mediated by chemosensory …

[HTML][HTML] Recent structural advances in bacterial chemotaxis signalling

C Riechmann, P Zhang - Current opinion in structural biology, 2023 - Elsevier
Bacterial chemosensory arrays have served as a model system for in-situ structure
determination, clearly cataloguing the improvement of cryo-electron tomography (cryoET) …

Progress and potential of electron cryotomography as illustrated by its application to bacterial chemoreceptor arrays

A Briegel, G Jensen - Annual Review of Biophysics, 2017 - annualreviews.org
Electron cryotomography (ECT) can produce three-dimensional images of biological
samples such as intact cells in a near-native, frozen-hydrated state to macromolecular …

CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling

CK Cassidy, BA Himes, FJ Alvarez, J Ma, G Zhao… - Elife, 2015 - elifesciences.org
Chemotactic responses in bacteria require large, highly ordered arrays of sensory proteins
to mediate the signal transduction that ultimately controls cell motility. A mechanistic …

Structure of the native chemotaxis core signaling unit from phage E-protein lysed E. coli cells

CK Cassidy, Z Qin, T Frosio, K Gosink, Z Yang… - Mbio, 2023 - Am Soc Microbiol
Motile bacteria employ conserved chemotaxis networks to detect chemical gradients in their
surroundings and effectively regulate their locomotion, enabling the location of essential …

Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response

CM Khursigara, G Lan, S Neumann, X Wu… - The EMBO …, 2011 - embopress.org
In chemotactic bacteria, transmembrane chemoreceptors, CheA and CheW form the core
signalling complex of the chemotaxis sensory apparatus. These complexes are organized in …

New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography

A Briegel, ML Wong, HL Hodges, CM Oikonomou… - Biochemistry, 2014 - ACS Publications
Bacterial chemoreceptors cluster in highly ordered, cooperative, extended arrays with a
conserved architecture, but the principles that govern array assembly remain unclear. Here …

Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

A Briegel, MS Ladinsky, C Oikonomou, CW Jones… - Elife, 2014 - elifesciences.org
Most motile bacteria sense and respond to their environment through a transmembrane
chemoreceptor array whose structure and function have been well-studied, but many …

Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations

CK Cassidy, BA Himes, D Sun, J Ma, G Zhao… - Communications …, 2020 - nature.com
To enable the processing of chemical gradients, chemotactic bacteria possess large arrays
of transmembrane chemoreceptors, the histidine kinase CheA, and the adaptor protein …