Figure of merit for CRISPR-based nucleic acid-sensing systems: Improvement strategies and performance comparison

R Nouri, M Dong, AJ Politza, W Guan - ACS sensors, 2022 - ACS Publications
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid-
sensing systems have grown rapidly in the past few years. Nevertheless, an objective …

Challenges and opportunities for clustered regularly interspaced short palindromic repeats based molecular biosensing

M Bao, Q Chen, Z Xu, EC Jensen, C Liu, JT Waitkus… - ACS …, 2021 - ACS Publications
Clustered regularly interspaced short palindromic repeats, CRISPR, has recently emerged
as a powerful molecular biosensing tool for nucleic acids and other biomarkers due to its …

CRISPR/cas systems redefine nucleic acid detection: principles and methods

M Wang, R Zhang, J Li - Biosensors and Bioelectronics, 2020 - Elsevier
Methods that enable rapid, sensitive and specific analyses of nucleic acid sequences have
positive effects on precise disease diagnostics and effective clinical treatments by providing …

[HTML][HTML] Challenges and perspectives of CRISPR-based technology for diagnostic applications

S Del Giovane, N Bagheri, AC Di Pede… - TrAC Trends in …, 2024 - Elsevier
The precision and versatility of CRISPR-based techniques, combined with the advantages of
nucleic acid-based nanotechnology, hold great promise in transforming the landscape of …

Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes–a review

S Xie, Z Ji, T Suo, B Li, X Zhang - Analytica Chimica Acta, 2021 - Elsevier
The CRISPR/Cas technology, derived from an adaptive immune system in bacteria, has
been awarded the Nobel Prize in Chemistry in 2020 for its success in gene editing …

Nucleic Acid Enzyme‐Activated CRISPR‐Cas12a With Circular CRISPR RNA for Biosensing

Y Wu, D Chang, Y Chang, Q Zhang, Y Liu, JD Brennan… - Small, 2023 - Wiley Online Library
Abstract clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas systems
are increasingly used in biosensor development. However, directly translating recognition …

The CRISPR/Cas system: a customizable toolbox for molecular detection

Y He, W Yan, L Long, L Dong, Y Ma, C Li, Y Xie, N Liu… - Genes, 2023 - mdpi.com
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated
proteins (Cas) are promising molecular diagnostic tools for rapidly and precisely elucidating …

Evolution of CRISPR-enabled biosensors for amplification-free nucleic acid detection

S Talebian, F Dehghani, PS Weiss, J Conde - Trends in Biotechnology, 2024 - cell.com
CRISPR biosensors enable rapid and accurate detection of nucleic acids without resorting
to target amplification. Specifically, these systems facilitate the simultaneous detection of …

Powerful CRISPR-based biosensing techniques and their integration with microfluidic platforms

B Chen, Y Li, F Xu, X Yang - Frontiers in bioengineering and …, 2022 - frontiersin.org
In the fight against the worldwide pandemic coronavirus disease 2019 (COVID-19), simple,
rapid, and sensitive tools for nucleic acid detection are in urgent need. PCR has been a …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …