Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

RE Veerman, L Teeuwen, P Czarnewski… - Journal of …, 2021 - Wiley Online Library
Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers,
but still EV subtypes are not fully characterised. To isolate EVs with few co‐isolated entities …

[HTML][HTML] Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents

A Gámez-Valero, M Monguió-Tortajada… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EVs) have become an attractive field among the scientific community.
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …

[HTML][HTML] Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization …

F Royo, C Théry, JM Falcón-Pérez, R Nieuwland… - Cells, 2020 - mdpi.com
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …

[HTML][HTML] Characterization of plasma-derived extracellular vesicles isolated by different methods: a comparison study

E Serrano-Pertierra, M Oliveira-Rodríguez, M Rivas… - Bioengineering, 2019 - mdpi.com
Extracellular vesicles (EV) are small membrane structures released by cells that act as
potent mediators of intercellular communication. The study of EV biology is important, not …

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion …

JA Martínez‐Greene… - Journal of …, 2021 - Wiley Online Library
The molecular characterization of extracellular vesicles (EVs) has revealed a great
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …

[HTML][HTML] Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation

EA Mol, MJ Goumans, PA Doevendans… - … , Biology and Medicine, 2017 - Elsevier
Extracellular vesicles (EVs) are nano-sized, lipid bilayer-enclosed particles involved in
intercellular communication. EVs are increasingly being considered as drug delivery …

[HTML][HTML] Commonly used methods for extracellular vesicles' enrichment: Implications in downstream analyses and use

M Clos-Sansalvador, M Monguió-Tortajada… - European Journal of …, 2022 - Elsevier
Extracellular vesicles (EVs) are becoming promising tools for clinical application, either as
sources of disease-specific molecular signatures for the unraveling of disease …

[HTML][HTML] Extracellular vesicle isolation: present and future

I Furi, F Momen-Heravi, G Szabo - Annals of translational medicine, 2017 - ncbi.nlm.nih.gov
In recent years, extracellular vesicle (EV)-based biomarker discovery has received
significant interest amongst scientist studying diverse disease conditions. Extracellular …

[HTML][HTML] Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography

N Heath, L Grant, TM De Oliveira, R Rowlinson… - Scientific reports, 2018 - nature.com
Extracellular vesicles (EVs) have important roles in physiology, pathology, and more
recently have been identified as efficient carriers of therapeutic cargoes. For efficient study of …

[HTML][HTML] Flow cytometry analysis of circulating extracellular vesicle subtypes from fresh peripheral blood samples

M Marchisio, P Simeone, G Bologna, E Ercolino… - International journal of …, 2020 - mdpi.com
Extracellular vesicles (EVs) are released by shedding during different physiological
processes and are increasingly thought to be new potential biomarkers. However, the …