Cellular growth and the preparation of cells for division between two successive cell
divisions is called the cell cycle. The cell cycle is divided into several phases; the length of …

Study of protein expression during the cell cycle requires preparation of pure fractions of
cells at various phases of the cell cycle. This was achieved by the development of methods …

Publisher Summary This chapter presents a discussion on cell-cycle analysis. In cell
biology, one of the most exciting and intriguing questions is the process of cell growth and …

The method of interpreting experimental data on labelled mitosis wave (Takahashi, 1966) is
reconfirmed using a less restrictive model which has the following characteristics: 1.(i) …

Flow cytometric multiparameter analysis of two proliferation-associated nuclear antigens
(proliferating cell nuclear antigen (PCNA)/cyclin and Ki-67) was performed on seven human …

Flow cytofluorimetric measurement of incorporated bromodeoxyuridine, using a double‐
stained cell population, allows the determination of the distribution of cells along the cell …

Multivariate analysis of the expression of cyclin proteins and DNA content has opened new
possibilities for the study of the cell cycle. By virtue of their cell cycle phase specificity, the …

Cyclins, the regulatory subunits of their respective cyclin-dependent kinases, are the key
components of the cell-cycle progression machinery. Some cyclins are expressed …

Simultaneous measurements of cellular DNA and RNA as well as estimates of the sensitivity
of DNA in situ to denaturation by acid (which correlates with the degree of chromatin …

Quantification of the proliferative characteristics of normal and malignant cells has been of
interest to oncolo gists and cancer biologists for almost three decades. This interest stems …